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October 4, 2013

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

ProtoLift Western Stripping Buffer

  • Strip PVDF blots in 10 minutes
  • Contains zero harsh detergents
  • Non-acidic
Catalog number: EC-889
The ProtoLift Western Stripping Buffer is a more effective system for stripping your PVDF Western Blots.  Antibodies can be stripped from blots within 10 minutes.  Target proteins are not stripped from the membrane.  Furthermore, blots can be stripped with ProtoLift Western Stripping Buffer multiple times without loss of signal.  Note: ProtoLift Western Stripping Buffer is not recommended for nitrocellulose blots.
 

ProtoLift does not remove target proteins
 

A PVDF blot of 8 identical lanes of mouse liver extract was divided in half, and lanes 5-8 were incubated in ProtoLift according to the standard protocol (see Figure 1).  Both membranes were stained with Coomassie Blue to detect total protein levels.  No detectable difference was seen between the control and the membrane incubated in ProtoLift Western Stripping Buffer.
 
Figure 1:  No detectable difference found between mouse liver extract lanes stripped with ProtoLift Western Stripping Buffer (Lanes 5-8) and control lanes (Lanes 1-4).
 

PVDF Blots can be stripped and reprobed multiple times with ProtoLift
 

A PVDF blot of mouse liver extract was probed for GAPDH, then stripped and reprobed 4 times (see Figure 2).  Panel A:  first probing, Panel B:  second probing, Panel C 4th probing.  All 4 probings produced identical signals.
 
Figure 2: Multiple strippings with ProtoLift Western Stripping Buffer cause minimal (if any) loss of detection.
 
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