Electrophoresis - Color Icons2

Electrophoresis

AquaPor ES GTAC Agarose

$63.00$226.00

Catalog number: EC-203
Quantity Discount when you buy 4 or more 100g units
  • Ultra-High Strength/Low EEO Agarose
  • Blotting of MegaBase DNA
  • Finest Particle Size for Fastest Dissolution

Description

Catalog number: EC-203
Quantity Discount when you buy 4 or more 100g units
  • Ultra-High Strength/Low EEO Agarose
  • Blotting of MegaBase DNA
  • Finest Particle Size for Fastest Dissolution
Catalog number: EC-203

The very low EEO of AquaPor ES will significantly reduce electrophoresis times for Pulsed Field Gel (PFG) applications, and also increase band sharpness. The extremely high gel strength makes it possible to cast gels as low as 0.3% which remain intact through staining, destaining, photographic documentation and blotting. These low percentage gels may be used for the electrophoretic separation of DNA fragments as large as 50 kb, and macromolecular protein complexes from 500 kDa to 10 MDa size.  Ultra-fine particle size ensures the fastest dissolution for easiest gel preparation.

EEO (-mr) Gel Strength (g/cm2) Gel Temp (°C)
≤ 0.05 ≥ 3200 @ 1.5% ≤ 40 @ 1.0%
Melt Temp (°C) Sulfate (%) DNase, RNase, Protease
≤ 89 @ 1.5% ≤ 0.20 None

Additional information

Weight 0.2 lbs
Dimensions 6 × 3 × 3 in

Protocol

DISSOLVING AQUAPOR AGAROSES
Catalog Number: EC-203: Download Full Protocol PDF >

A. Add room temperature buffer to a flask that is 2.5 – 4 times the volume of gel solution. Add a teflon-coated stir bar.
B. Add AquaPor powder while stirring vigorously so the agarose is dispersed uniformly. Stir for 2 minutes to hydrate the agarose.
C. Tare the flask and solution.
D. Place in a microwave oven and heat at 100% power using 20 – 60 second intervals. Swirl gently between intervals to resuspend the agarose.
E. Continue the cycle of heating and swirling until the agarose is completely dissolved (no visible particles are present).
F. Add distilled water to obtain the initial weight and mix.
G. Cool the solution to 50 – 60°C before pouring the gel.

* Chill the gel for 30 minutes prior to comb removal when using AquaPor LM, HR, and low (<1%) concentration of AquaPor LE and ES.

Catalog Number:
EC-202
EC-203
EC-204
EC-205
EC-206

Safety Overview

Appearance and Odor
White powder

EMERGENCY OVERVIEW – IMMEDIATE HAZARD

AS PART OF GOOD INDUSTRIAL AND PERSONAL HYGIENE AND SAFETY PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.

Full MSDS Protocols

Catalog number: EC-203
DOWNLOAD MSDS PDF >