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Electrophoresis

SequaGel UreaGel 19:1 Denaturing Gel System

$113.00$183.00

Quantity Discount when you buy 4 or more 2.2 liter kit
Catalog number: EC-833
  • Casts 19:1 Denaturing Gels up to 20% Monomer
  • Certified RNase and DNase Free
  • Consistently Crystal Clear Gels
  • Twelve Month Shelf Life at Room Temperature

Description

Quantity Discount when you buy 4 or more 2.2 liter kit
Catalog number: EC-833
  • Casts 19:1 Denaturing Gels up to 20% Monomer
  • Certified RNase and DNase Free
  • Consistently Crystal Clear Gels
  • Twelve Month Shelf Life at Room Temperature
The UreaGel System consists of UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the UreaGel System, the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 acrylamide: bisacrylamide). Liter bottles of UreaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution. UreaGel Diluent is supplied in 450 ml and 1-liter bottles containing 7.5M urea in deionized water. UreaGel Buffer is supplied in 100 ml and 200 ml bottles containing 0.89M Tris-Borate-20mM EDTA buffer pH 8.3 (10X TBE) and 7.5M urea.
Storage: UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer are stable for one year when stored tightly capped, in a dark area at room temperature (20°C).

Additional information

Weight 5 lbs
Dimensions 14 × 7 × 18 in

Protocol

Download Full Protocol PDF >

Mix UreaGel System Components:
Determine how much UreaGel Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix.

For 100 ml Gel Casting Solution:
Vc =(Vt) (X)/25
Vb = 0.1 (Vt)
Vd = Vt – (Vc + Vb)

where:
Vc = UreaGel Concentrate Volume
Vb = UreaGel Buffer Volume
Vd = UreaGel Diluent Volume
Vt = Total Casting Solution Volume
X = % Gel Desired

EXAMPLE:

To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows:
Vc =(100) (8)/25 = 32ml UreaGel Concentrate
Vb = 0.1 (100) = 10ml UreaGel Buffer
Vd = 100 – (32 + 10) = 58ml UreaGel Diluent

Add Initiators and Cast Gel:
Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.

Catalog Number:
EC-833
EC-830
EC-835
EC-840

Safety Overview

Safety Summary (see MSDS for complete information before using product):

UreaGel 29:1 Concentrate Safety Overview

Catalog Number: EC-835

Appearance and Odor
Clear colorless solution
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
Urea
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR INHALED.
Boric Acid
CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. BORIC ACID IS HARMFUL IF SWALLOWED OR INHALED.
Tris-Base
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. HARMFUL IF SWALLOWED OR INHALED.


Catalog Number: EC-830

Appearance and Odor:
Clear colorless solution.

May cause cancer. May cause heritable genetic damage. Also toxic in contact with skin and if swallowed. Danger of serious damage to health by prolonged exposure through inhalation, in contact with skin or if swallowed.
Avoid exposure, obtain special instructions before use. In case of accident or if you feel ill, seek medical advice immediately (show the label where possible).
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
WARNING! HARMFUL IF SWALLOWED. ACRYLAMIDE IS A NEUROTOXIN. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION.


Catalog Number: EC-840

Appearance and Odor
Clear, colorless solution

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR INHALED.

Full MSDS Protocols

Full MSDS PDF: EC-835 >

Full MSDS PDF: EC-830 >

Full MSDS PDF: EC-840 >