Electrophoresis
SequaGel UreaGel 19:1 Denaturing Gel System
Price range: $136.00 through $221.00
- Casts 19:1 Denaturing Gels up to 20% Monomer
- Certified RNase and DNase Free
- Consistently Crystal Clear Gels
- Twelve Month Shelf Life at Room Temperature
Description
- Casts 19:1 Denaturing Gels up to 20% Monomer
- Certified RNase and DNase Free
- Consistently Crystal Clear Gels
- Twelve Month Shelf Life at Room Temperature
Additional information
| Weight | 5 lbs |
|---|---|
| Dimensions | 14 × 7 × 18 in |
Protocol
Procedure
Mix UreaGel System Components
Determine how much UreaGel Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix.
For 100 ml Gel Casting Solution:
Vc =(Vt) (X)/25
Vb = 0.1 (Vt)
Vd = Vt – (Vc + Vb)
where:
Vc = UreaGel Concentrate Volume
Vb = UreaGel Buffer Volume
Vd = UreaGel Diluent Volume
Vt = Total Casting Solution Volume
X = % Gel Desired
EXAMPLE:
To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows:
Vc =(100) (8)/25 = 32ml UreaGel Concentrate
Vb = 0.1 (100) = 10ml UreaGel Buffer
Vd = 100 – (32 + 10) = 58ml UreaGel Diluent
Add Initiators and Cast Gel
Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.
Safety Overview
Safety Summary (see SDS for complete information before using product):
UreaGel 19:1 Denaturing Gel System Safety Overview
UreaGel Concentrate
Appearance and Odor
Clear colorless solution
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
Urea
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR INHALED.
Boric Acid
CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. BORIC ACID IS HARMFUL IF SWALLOWED OR INHALED.
Tris-Base
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. HARMFUL IF SWALLOWED OR INHALED.
Appearance and Odor:
Clear colorless solution.
May cause cancer. May cause heritable genetic damage. Also toxic in contact with skin and if swallowed. Danger of serious damage to health by prolonged exposure through inhalation, in contact with skin or if swallowed.
Avoid exposure, obtain special instructions before use. In case of accident or if you feel ill, seek medical advice immediately (show the label where possible).
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
WARNING! HARMFUL IF SWALLOWED. ACRYLAMIDE IS A NEUROTOXIN. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION.
UreaGel Buffer
Appearance and Odor
Clear, colorless solution
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR INHALED.
Full SDS
UreaGel Concentrate
View full SDS >
UreaGel Diluent
View full SDS >
UreaGel Buffer
View full SDS >
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