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Electrophoresis

SequaGel UreaGel 19:1 Concentrate

$68.00$125.00

Quantity Discount when you buy 4 or more 1 liter units
 
Catalog Number: EC-830
UreaGel Concentrate is one of three components of the UreaGel System. The UreaGel System (formerly SequaGel Sequencing System) consists of UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the UreaGel System the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 Acrylamide/Bisacrylamide). Liter bottles of UreaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution.

Description

Quantity Discount: 10% discount on purchase of 4 or more 1 liter units Catalog Number: EC-830

UreaGel Concentrate is one of three components of the UreaGel System. The UreaGel System (formerly SequaGel Sequencing System) consists of UreaGel Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare gels of varying percentage. With the UreaGel System the researcher readily prepares any commonly used gel formulation up to 20% monomer (19:1 Acrylamide/Bisacrylamide). Liter bottles of UreaGel Concentrate contain 237.5 grams of acrylamide, 12.5 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution.

Additional information

Weight 2.5 lbs
Dimensions 7 × 7 × 14 in

Protocol

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Mix UreaGel System Components: Determine how much UreaGel Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix. For 100 ml Gel Casting Solution: Vc =(Vt) (X)/25 Vb = 0.1 (Vt) Vd = Vt – (Vc + Vb) where: Vc = UreaGel Concentrate Volume Vb = UreaGel Buffer Volume Vd = UreaGel Diluent Volume Vt = Total Casting Solution Volume X = % Gel Desired EXAMPLE: To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows: Vc =(100) (8)/25 = 32ml UreaGel Concentrate Vb = 0.1 (100) = 10ml UreaGel Buffer Vd = 100 – (32 + 10) = 58ml UreaGel Diluent Add Initiators and Cast Gel: Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.
Catalog Number:
EC-833
EC-830
EC-835
EC-840

Safety Overview

Safety Summary (see MSDS for complete information before using product):

Appearance and Odor: Clear colorless solution.

May cause cancer. May cause heritable genetic damage. Also toxic in contact with skin and if swallowed. Danger of serious damage to health by prolonged exposure through inhalation, in contact with skin or if swallowed. Avoid exposure, obtain special instructions before use. In case of accident or if you feel ill, seek medical advice immediately (show the label where possible). EMERGENCY OVERVIEW – IMMEDIATE HAZARD WARNING! HARMFUL IF SWALLOWED. ACRYLAMIDE IS A NEUROTOXIN. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION.

Full MSDS Protocols

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