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Electrophoresis

ProtoStain Blue

$93.00

Catalog Number: EC-727
Size: 1 liter

Get a free sample of ProtoStain Blue! Call us at (800) 526-3867 for details!

    • The faster, more sensitive colloidal Coomassie Blue stain
    • Bands appear in 15 minutes
    • Safe, non-hazardous formula

Description

Catalog Number: EC-727
Size: 1 liter

Get a free sample of ProtoStain Blue! Call us at (800) 526-3867 for details!

    • The faster, more sensitive colloidal Coomassie Blue stain
    • Bands appear in 15 minutes
    • Safe, non-hazardous formula

    ProtoStain Blue is a colloidal Coomassie Blue G-250 solution that is a safer, more sensitive method for detecting proteins on electrophoresis gels.   Bands begin to appear in only 15 minutes, and ProtoStain Blue has the ability to detect down to 1ng denatured BSA.

    ProtoStain Blue is safe and nonhazardous, containing no methanol, acetic acid or phosphoric acid.  Where local regulations permit, ProtoStain Blue can be poured down the drain, as it is not classified as a hazardous waste according to United States Title 40 Code of Federations (40 CFR 261.24 (a)) and EEC Directive 79/831/EEC Annex VII, JIS K 3363-1967 (always consult local and state regulations before disposing of any laboratory reagents).

Additional information

Weight 4.6 lbs

Protocol

Download Full Protocol >

Note: This protocol is optimized for a 0.75mm thick gel. Thicker gels require longer water
washes.
To conserve solution, use a plastic container.
    1. Wash gel 3 times at 10 minutes each with deionized water on an orbital shaker. Decant wash solution. Longer washes give better consistency.

 

    1. After the last wash, add enough ProtoStain Blue solution to completely cover the gel. Invert bottle before use to resuspend particles before dispensing stain solution.

 

    1. Bands containing more than 1μg of protein will be detected within 15 minutes. For full sensitivity incubate the gel in the stain for at least 4-5 hours. Longer incubations in the stain will not adversely affect the gel or the staining sensitivity.

 

  1. Remove the stain and wash the gel in deionized water. Incubating the gel in water increases the sensitivity of detection by reducing the background to crystal clear. The gel is stable in water for up to a week without loss of sensitivity. There is no need to store the gel in a salt solution.

Safety Overview

Safety Summary (see MSDS for complete information before using product):

Appearance and odor
Odorless, colorless solution.

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
None

Full MSDS Protocols

Catalog number: EC-727
DOWNLOAD MSDS PDF >