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Electrophoresis

ProtoGel Resolving Buffer (4X)

$40.00$70.00

Quantity Discount when you buy 4 or more 1 Liter units

Catalog number: EC-892

  • Traditional Laemmli Resolving Gel Buffer (4X)
  • Formulated with 0.2 Micron Filtration
  • Ultra-Pure Reagents in 18 MegOhm Water
  • Crystal Clear, Reproducible Gels

Description

Quantity Discount when you buy 4 or more 1 Liter units

Catalog number: EC-892

  • Traditional Laemmli Resolving Gel Buffer (4X)
  • Formulated with 0.2 Micron Filtration
  • Ultra-Pure Reagents in 18 MegOhm Water
  • Crystal Clear, Reproducible Gels

The use of National Diagnostics’ ProtoGel Resolving Buffer will ensure the purity and performance of your Laemmli gels. When diluted as directed ProtoGel Resolving Buffer forms a gel of 0.375 M Tris-HCl and 0.1% SDS, pH 8.8.

Storage: ProtoGel Resolving Buffer is stable for 24 months when stored tightly capped in a dark area at room temperature.

Additional information

Weight 2.8 lbs
Dimensions 7 × 7 × 14 in

Protocol

Measure and Mix Solutions:
The volume of ProtoGel required for gel casting solutions of any volume and acrylamide concentration may be calculated from the following formula:
Vp =(X) (Vt)/40
where:
Vp = Volume of 40% ProtoGel
X = % Monomer Desired in Gel
Vt = Total Volume of Gel Casting Solution

EXAMPLE: To make 100 ml of a 10% monomer gel, calculate the volume of Protogel to add as follows:
Vp = (10) (100)/40 = 25.0 ml

Initiate and Cast Gel:
For optimal results degas gel solution for 10 minutes under vacuum aspiration prior to innitiation with APS and TEMED. Add 1.0ml of 10% (w/v) ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1 ml of TEMED for every 100ml of gel casting solution. Swirl gently to mix. Pour the solution into the gel casting cassette. The gel should begin to set in 10-20 minutes. To provide a sharp interface, overlay the gel with water saturated  n-butanol during polymerization. Flush butanol away with water just before casting the stacking gel (below).

Cast Stacking Gel:
Use ProtoGel Stacking Buffer to make 10ml of a 4% stacking gel:

  • ProtoGel: 1.00ml
  • ProtoGel Stacking Buffer: 2.50ml
  • Deionized water: 6.44ml

Add 0.05ml 10% ammonium persulfate and 0.01ml of TEMED. Gel will begin to set in 20 minutes.

Safety Overview

Safety Summary (see MSDS for complete information before using product):

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
WARNING! HARMFUL IF SWALLOWED. ACRYLAMIDE MAY CAUSE NERVOUS SYSTEM DAMAGE. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION.

Full MSDS Protocols

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