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Electrophoresis

Nuclistain

$89.00

Quantity Discount when you buy 4 or more bottles

Catalog Number: EC-730

Size: 25 ml
  • UV Free Visualization of DNA and RNA
  • Improves Yield in PCR Purification from Agarose Gels
  • Easy and Safe

Description

Quantity Discount when you buy 4 or more bottles

Catalog Number: EC-730

Size: 25 ml
  • UV Free Visualization of DNA and RNA
  • Improves Yield in PCR Purification from Agarose Gels
  • Easy and Safe

Nuclistain improves the yield of smaller and medium sized DNA (<500 bp) obtained by purification from agarose. One gel was stained with Nuclistain and the other with EtBr. The DNA was subsequently purified from each and then run side by side on a third gel, visualized by EtBr. The results show the improved yield from the Nuclistain.

Nuclistain is intended as a replacement for the conventionally used ethidium bromide for the rapid detection of double and single-stranded DNA and RNA in agarose and polyacrylamide gels. Its sensitivity is nearly comparable, with the capability of detecting as little as 10-50 ng of nucleic acid sample. The advantage of Nuclistain is that the results are visible under normal lighting conditions, eliminating the possibility of sample damage by UV radiation. Furthermore, Nuclistain visualization dramatically improves the yield of smaller and medium-sized DNA (<500 bp) obtained by purification from agarose. Nuclistain binding is reversible and does not modify nucleic acids.

Nuclistain is extremely easy to use. One 25 ml bottle of Nuclistain will make 2.5 liters of stain solution. The separated DNA appears as dark blue bands on a light blue background.

Additional information

Weight 0.3 lbs
Dimensions 6 × 3 × 3 in

Protocol

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  1. To prepare a working strength solution of NucliStain, add one part NucliStain concentrate to one-hundred parts of distilled water. Mix until the solution is uniform. Prepare sufficient stain solution to fully submerge the gel.
  2. Immerse the gel in the stain solution for 20–30 minutes.
  3. Immerse the gel in distilled water for 3–4 hours to destain the blue background. Bands can often be visualized minutes after destaining. However, for increased sensitivity, overnight destaining is recommended.

Safety Overview

Safety Summary (see MSDS for complete information before using product):
Appearance and Odor
Opaque blue liquid.

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
MAY BE HARMFUL IF SWALLOWED.
EMERGENCY OVERVIEW – CHRONIC HAZARD WARNING
LABORATORY EXPERIMENTS HAVE SHOWN MUTAGENIC EFFECTS.

Full MSDS Protocols

Catalog Number: EC-730
DOWNLOAD MSDS PDF >