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Electrophoresis

Insite Markers

$129.00

Catalog number: EC-897
Size: 0.5 ml
  • Two Sets of Markers in One
  • Prestained Markers Provide Orientation During the Run
  • Engineered Molecular Weight Standards Appear in Fluorescent Detection

Description

Catalog number: EC-897
Size: 0.5 ml
  • Two Sets of Markers in One
  • Prestained Markers Provide Orientation During the Run
  • Engineered Molecular Weight Standards Appear in Fluorescent Detection

National Diagnostics’ Insite Markers contain both visible markers for orientation during the run and high precision protein standards (10-225 kD) that appear with fluorescent detection. This allows both confident monitoring of the run and precise assignment of protein molecular weights.

The Insite Markers are supplied in a 0.5 mL vial. Each vial contains sufficient material to stain between 50 and 100 mini-gels.

Additional information

Weight 4 lbs
Dimensions 8 × 6 × 6 in

Protocol

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Twenty Minute Casting

ProtoGel Quick-Cast contains the monomers and buffer components to produce a 12% gel.

  1. Measure out the volume of ProtoGel Quick-Cast needed to fill the cassette – typically 10ml for one mini-gel, 15ml for two.
  2. Add 100 microliters of fresh 10% APS and 10 microliters of TEMED per 10ml ProtoGel Quick-Cast. Mix briefly and pour into the gel cassette.
  3. 3. Insert comb and allow to polymerize at room temperature for 20 minutes. The gel is now ready to run.

For best results, it is recommended you use ProtoGel Quick-Cast Loading Buffer. Simply mix your samples with an equal volume of ProtoGel Quick-Cast Loading Buffer, load and run.

Full MSDS Protocol

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