Version 3.0
Date revised: 4/26/2019

SAFETY DATA SHEETNational Diagnostics

Conforms to regulation (EC) no. EU 453/2010

SECTION 1 - IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING

1.1 Product Identifier

Product Name: Insite Markers Product Number: EC-897

1.2 Relevant Identified Uses of the Substance/Mixture and Uses Advised Against

Investigational research by professional users

1.3 Details of the Supplier of the Safety Data Sheet

Manufacturer
National Diagnostics
305 Patton Drive
Atlanta, GA 30036
(404) 699-2121
(800) 526-3867
info@nationaldiagnostics.com

1.4 Emergency Telephone Number

ChemTel Inc.
Contract number MIS8894340
1-800 255-3924 (United States, Canada, Puerto Rico & US Virgin Islands)
01-800-099-0731 (Mexico)
400-120-0751 (China)
000-800-100-4086 (India)
1-300-954-583 (Australia)
0-800-591-6042 (Brazil)
+1-813-255-3924 (All other regions)

SECTION 2 - HAZARDS IDENTIFICATION

2.1 Classification of the Substance or Mixture

Classification according to Regulation (EC) No. 1272/2008 [EU-GHS/CLP]

H302 - Acute Toxicity-Oral (Category 4)
H312 - Acute Toxicity-Dermal (Category 4)
H315 - Skin Corrosion/Irritation (Category 2)
H319 - Serious Eye Damage/Eye Irritation (Category 2A
H332 - Acute Toxicity-Inhalation (Category 4)

2.2 Label Elements

GHS LABEL ELEMENTS AND CLASSIFICATION
GHS Label Elements
Harmful 

WARNING

H302 - Harmful if swallowed.
H312 - Harmful in contact with skin.
H315 - Causes skin irritation.
H319 - Causes serious eye irritation. H332 - Harmful if inhaled.
P262 - Do not get into eyes, on skin or on clothing.
P264 - Wash skin thoroughly after handling.
P280 - Wear protective gloves/protective clothing/eye protection/face protection.
P301+P312 - IF SWALLOWED: Immediately call a POISON CENTER or doctor/physician IF you feel unwell.
P302+P352 - IF ON SKIN: Wash with plenty of soap and water.
P305+P351+P338 - IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses if present and easy to do. Continue rinsing.
P304+P341 - IF INHALED: If breathing is difficult, remove victim to fresh air and keep at rest in a position comfortable for breathing.

  
  

2.3 Other Hazards

None found.

SECTION 3 - COMPOSITION/INFORMATION ON INGREDIENTS

3.2 Mixture

Chemical Names/Description

Aqueous solution of tris base, SDS, glcyerol, mercaptoethanol (and dyed proteins <1%)


Component List

Component % Comp. CAS # EC # 1278/2008 Classification
2-Mercaptoethanol2 60-24-2  200-464-6 H301, H310, H315, H317, H318, H331, H373, H400, H410
Tris-Base1.5 77-86-1  201-064-4 H315, H319, H335
SDS4.4 151-21-3   205-788-1 H302, H315, H319, H335
Glycerol20 56-81-5  200-289-5 N.A.


SECTION 4 - FIRST AID MEASURES

4.1 Description of First Aid Measures

Inhalation

Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Call a physician.

Ingestion

Do not induce vomiting. If swallowed and the person is conscious, immediately give large amounts of water. Get medical attention.

Skin

Immediately flush skin with plenty of soap and water for at least 15 minutes while removing contaminated clothing and shoes. Get medical attention. Wash clothing before reuse. Thoroughly clean shoes before reuse.

Eyes

Immediately flush eyes with plenty of water for at least fifteen minutes, lifting lower and upper eyelids occasionally. Get medical attention immediately.

4.2 Most Important Symptoms and Effects, Both Acute and Delayed

Inhalation

2-Mercaptoethanol:

Symptoms may include coughing, sore throat, shortness of breath, headaches, nausea, and vomiting. Prolonged exposure can cause CNS stimulation.

Tris-Base:

Coughing, shortness of breath.

SDS:

Coughing, shortness of breath. May cause allergic reaction in sensitive individuals.

Glycerol:

Inhalation symptoms unlikely.

Ingestion

2-Mercaptoethanol:

Symptoms may include sore throat, abdominal pain, and vomiting.

Tris-Base:

Symptoms may include nausea, vomiting, and diarrhea. Large oral doses may cause weakness, collapse, blood clotting, and coma. The estimated lethal dose of Tris Base is 50 grams dry solid.

SDS:

Nausea and diarrhea.

Glycerol:

Nausea, headache, diarrhea.

Skin

2-Mercaptoethanol:

Symptoms may include skin irritation.

Tris-Base:

Redness, itching, and pain.

SDS:

Causes dryness and a rash on continued exposure.

Glycerol:

Irritation.

Eyes

2-Mercaptoethanol:

Symptoms may include redness and pain.

Tris-Base:

Redness, itching, and pain.

SDS:

Causes redness and pain.

Glycerol:

Irritation.

4.3 Indication of Any Immediate Medical Attention and Special Treatment Needed

Unknown/not applicable

SECTION 5 - FIRE FIGHTING MEASURES

5.1 Extinguishing media

Use media appropriate to the primary cause of fire.

5.2 Special Hazards Arising from the Substance/Mixture

Hazardous Combustion Products

Thermal decomposition products may include toxic oxides of nitrogen, sulfur, and carbon.

Hazardous Decomposition Products

Toxic gases may be involved in a fire. Glycerin decomposes upon headting above 290C, forming corrosive gas (acrolein).

Hazardous Polymerization

Will not occur under normal conditions of use (See Sections 10.4 & 10.5).

5.3 Advice for Firefighters

In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full facepiece operated in the pressure demand or other positive pressure mode.

5.4 Further Information

No data available.

SECTION 6 - ACCIDENTAL RELEASE MEASURES

6.1 Personal Precautions

Wear appropriate protective equipment as specified in Section 8.

6.2 Environmental Precautions

Prevent discharge into the environment. Dike spills and stop leakage where practical. Do not allow material to enter drains.

6.3 Methods and Materials for Containment and Cleaning Up

Contain and clean up spill immediately, prevent from entering floor drains. Contain liquids using absorbents. Shovel all spill materials into disposal drum. Scrub spill area with detergent, flush with copious amounts of water.

6.4 References to Other Sections

For disposal information see Section 13. For protective clothing and equipment see Section 8.

SECTION 7 - HANDLING AND STORAGE

7.1 Precautions for Safe Handling

Avoid contact and inhalation. Do not get in eyes, on skin, on clothing. Wash thoroughly after handling.

7.2 Conditions for Safe Storage (including any incompatibles)

Keep frozen until ready to use. Protect from physical damage. Isolate from incompatible materials (section 10).

Incompatibles

2-Mercaptoethanol:

Oxidizing agents, moisture, Avoid contact with metals.

Tris-Base:

No incompatibility data found.

SDS:

Strong oxidizers, acids.

Glycerol:

Strong oxidizers. Can react violently with acetic anhydride, calcium oxychloride, chromium oxides and alkali metal hydrides.

7.3 Specific End Uses

Investigational research by professional users

SECTION 8 - EXPOSURE CONTROLS/PERSONAL PRECAUTIONS

8.1 Control Parameters

Component: 2-Mercaptoethanol

ACGIH Threshold Limit Value (TLV):

Not Established

OSHA Permissable Exposure Limit (PEL):

AIHA WEEL 0.2ppm, 8 hr. TWA

Component: Tris-Base

ACGIH Threshold Limit Value (TLV):

none established

OSHA Permissable Exposure Limit (PEL):

none established

Component: SDS

ACGIH Threshold Limit Value (TLV):

None established

OSHA Permissable Exposure Limit (PEL):

None established

Component: Glycerol

ACGIH Threshold Limit Value (TLV):

10 mg/m3

OSHA Permissable Exposure Limit (PEL):

None established

8.2 Exposure Controls

Engineering Controls

A system of local and/or general exhaust is recommended to keep employee exposures below the Airborne Exposure Limits. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source.

Respiratory Protection

If the exposure limit is exceeded, wear a supplied air, full-facepiece respirator, airlined hood, or full-facepiece self-contained breathing apparatus.

Eye Protection

Use chemical safety goggles and/or a full face shield where splashing is possible. Maintain eye wash fountain and quick-drench facilities in work area.

Skin Protection

Wear impervious protective clothing, including boots, gloves, lab coat, apron or coveralls, as appropriate, to prevent skin contact.


SECTION 9 - PHYSICAL AND CHEMICAL PROPERTIES

9.1 Information on Basic Physical & Chemical Properties

a. AppearanceClear, colorless solutionb. OdorNone
c. Odor ThresholdN.A.d. pH6.8
e. Melting/Freezing Point (oC)-5f. Boiling point (oC)107
g. Flash Point (oC)N.A.h. Evaporation RateWater
i. FlammabilityN.A.j. Upper/Lower Flammability or Explosive LimitsN.A.
k. Vapor PressureWaterl. Vapor Density (Air = 1)Water
m. Relative DensityNo information.n. Water SolubilitySoluble
o. Partition Coefficient n-octanol/watermixturep. Autoignition Temperature (oC)N.A.
q. Decomposition Temperature (oC)Not applicable.r. ViscosityNo data available.
s. Explosive PropertiesN.A.t. Oxidizing PropertiesNot an oxidizer

SECTION 10 - STABILITY AND REACTIVITY

10.1 Reactivity

Non reactive under normal conditions of use and storage.

10.2 Chemical Stability

Stable under recommended conditions of use and storage.

10.3 Possibility of Hazardous Reactions

Will not occur under normal conditions of use (See Sections 10.4 & 10.5).

10.4 Conditions to Avoid

Heat, incompatibles.

10.5 Incompatible Materials

2-Mercaptoethanol:

Oxidizing agents, moisture, Avoid contact with metals.

Tris-Base:

No incompatibility data found.

SDS:

Strong oxidizers, acids.

Glycerol:

Strong oxidizers. Can react violently with acetic anhydride, calcium oxychloride, chromium oxides and alkali metal hydrides.

10.6 Hazardous Decomposition Products

Toxic gases may be involved in a fire. Glycerin decomposes upon headting above 290C, forming corrosive gas (acrolein).

SECTION 11 - TOXICOLOGICAL INFORMATION

Product LD50 Values

Oral Rat LD50 (mg/kg)

12200

Dermal Rabbit LD50 (mg/kg)

7500

Component Cancer List Status

 NTP Carcinogen 
 KnownAnticipatedIARC Category
2-MercaptoethanolNoNoNone
Tris-BaseNoNoNone
SDSNoNoNone
GlycerolNoNoNone

Potential Health Effects

Inhalation

2-Mercaptoethanol

Vapors irritate the mucous membranes and respiratory tract.

Tris-Base

Causes irritation to the respiratory tract.

SDS

Dust causes irritation to the respiratory tract.

Glycerol

Due to the low vapor pressure, inhalation of the vapors at room temperatures in unlikely. Inhalation of mist may cause irritation of respiratory tract.

Ingestion

2-Mercaptoethanol

Toxic. Harmful if swallowed. Sore throat, abdominal pain and vomiting may occur.

Tris-Base

Causes irritation and reddening to the mucous membranes of the mouth, esophagus, and gastrointestinal tract.

SDS

Large doses may cause gastrointestinal distress.

Glycerol

Low toxicity. May cause nausea, headache, diarrhea.

Skin

2-Mercaptoethanol

Toxic. Causes skin irritation and may be absorbed in the body in toxic quantities.

Tris-Base

Causes irritation to the skin.

SDS

Mildly irritating to skin. May cause allergic skin reactions.

Glycerol

May cause irritation.

Eyes

2-Mercaptoethanol

Vapors irritate the eyes with redness and pain. Splashes may cause severe irritation.

Tris-Base

Causes irritation to the eyes.

SDS

Causes irritation to the eyes.

Glycerol

May cause irritation.

Carcinogenicity

2-Mercaptoethanol

Substance is neither a known nor an anticipated carcinogen. Not listed by NTP, IARC, or OSHA.

Tris-Base

Not listed as a carcinogen by NTP or IARC.

SDS

Not listed as a known or suspected carcinogen by NTP or IARC.

Glycerol

According to definitions of the U.S. Hazard Communication Standard and the Canadian WHMIS Regulation, this material is not listed on the NTP, IARC, ACGIH, or OSHA carcinogen lists, and there are no studies implicating components as cancer causing agents in humans or animals.

Mutagenicity

2-Mercaptoethanol

No information available.

Tris-Base

No information found.

SDS

Has caused mutagenic effects on laboratory animals.

Glycerol

No information found.

Reproductive Toxicity

2-Mercaptoethanol

No information available.

Tris-Base

No information found.

SDS

Has caused mutagenic effects on laboratory animals.

Glycerol

No information found.

Teratogenic Effects

2-Mercaptoethanol

No information available.

Tris-Base

No information found.

SDS

No information found.

Glycerol

No information found.

Routes of Entry

2-Mercaptoethanol

Toxic effects possible by inhalation, ingestion, and skin absorption.

Tris-Base

Ingestion.

SDS

No information found.

Glycerol

No information found.

Target Organ Statement

2-Mercaptoethanol

Behavioral: Tremor, convulsion, excitement, muscle contraction/spasticity. Lungs, thorax: Respiratory depression. GI: Changes in structure/function of salivary glands.

Tris-Base

No information available.

SDS

Persons with pre-existing skin disorders or impaired respiratory function may be more susceptible to the effects of the substance.

Glycerol

Persons with pre-existing skin disorders or eye problems or impaired liver or kidney function may be more susceptible to the effects of this substance.

SECTION 12 - ECOLOGICAL INFOMATION

12.1 Toxicity

COMPONENT: 2-Mercaptoethanol

 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
LC50 (96hr, golden orfe) 37 mg/LEC50 (daphnia, 48 hr) 0.4 mg/LLC50 (96h) : 19 mg/LEC50 (17 h) : 113 mg/l

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data

COMPONENT: Tris-Base

 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
LC50 460mg/l (Golden ide)EC50: 59.8 mg/L (Daphnia)EC50: 473mg/l @ 48 hrsCE50>1000mg/L (3hrs)

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data

COMPONENT: SDS

 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
The 96 hr LC50 of dodecyl sulfate to Fathead minnows was 29 mg/LLC50 (Ceriodaphnia dubia, 48-hr): 5.55 mg/LEC50>120mg/LIC50 (3 hrs): 480 mg/L

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataEC50 (72hr, Cicer arietinum ) 361 mg/LNo data

COMPONENT: Glycerol

 VertebratesInvertebratesAlgaeMicroorganisms
Aquatic Toxicity
(ppm unless otherwise noted)
No dataNo dataEC3 : >10,000 mg/LEC50 (16hrs) > 10000 mg/L

 BirdsArthropodsPlantsMicroorganisms
Terrestrial Environment Toxicity
(ppm unless otherwise noted)
No dataNo dataNo dataNo data

12.2 Persistence and Degradability

2-Mercaptoethanol

Biodegradable (90% in 28 days)

Tris-Base

Readily Biodegradable (>97% degradation at 28 days)

SDS

Readily biodegradable (>95% degradation in 28 days)

Glycerol

Readily biodegradable

12.3 Bioaccumulative Potential

2-Mercaptoethanol

No data

Tris-Base

No data

SDS

No data

Glycerol

No data

12.4 Mobility in Soil

2-Mercaptoethanol

Koc 1.325

Tris-Base

Log Koc 1.57-1.85

SDS

Log Koc 1.545

Glycerol

No data

12.5 Results of PBT and vPvB Assessment

2-Mercaptoethanol

Not a PBT or vPvB

Tris-Base

Not a PBT or vPvB

SDS

Not PBT vPvB

Glycerol

Not PBT or vPvB

12.6 Other Adverse Effects

2-Mercaptoethanol

None

Tris-Base

None

SDS

None

Glycerol

None


SECTION 13 - DISPOSAL CONSIDERATIONS

13.1 Waste Treatment Methods

Offer surplus or non-recyclable product to licensed disposal company. Disposal is subject to user compliance with applicable law and product characteristics at time of disposal. Dispose of packaging as product.


SECTION 14 - TRANSPORT INFORMATION

  ADR/RID IATA IMO DOT
14.1 UN Number N.A.N.A.N.A.N.A.
14.2 Shipping NameNot regulated.Not regulated.Not regulated.Not regulated.
14.3 Hazard ClassN.A.N.A.N.A.N.A.
14.4 Packing GroupN.A.N.A.N.A.N.A.
14.5 Environmental HazardsN.A.N.A.Not regulatedN.A.
14.6 Special PrecautionsN.A.N.A.N.A.N.A.

SECTION 15 - REGULATORY INFORMATION

15.1 Safety, Health and Environmental Regulations/Legislation Specific for the Substance/Mixture

United States

TSCA Regulatory Statement

All intentional ingredients are listed on the TSCA Inventory.

SARA 311/312 Hazard Categories
ComponentFirePressureReactivityAcuteChronic
2-MercaptoethanolYesNoNoYesNo
Tris-BaseNoNoNoYesNo
SDSNoNoNoYesYes
GlycerolNoNoNoYesYes

Europe

EEC Regulatory

All intentional ingredients are listed on the European EINECS Inventory.

SECTION 16 - OTHER INFORMATION

Revisional Updates

4/26/2019 - Updated Section 1.4
5/29/2015 - Updated Sections 2.1 and 3.2
12/12/2013 - Released Version 1.0

NFPA Codes

Health N.D.   Flammability N.D.  Reactivity N.D.

Dangers

2-Mercaptoethanol

H301 - Toxic if swallowed
H310 - Fatal in contact with skin.
H315 - Causes skin irritation.
H317 - May cause an allergic skin reaction.
H318 - Causes serious eye damage.
H331 - Toxic if inhaled.
H373 - May cause damage to organs through prolonged or repeated exposure.
H401 - Toxic to aquatic life.
H410 - Very toxic to aquatic life with long lasting effects.

Tris-Base

H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.

SDS

H302 - Harmful if swallowed
H315 - Causes skin irritation.
H319 - Causes serious eye irritation.
H335 - May cause respiratory irritation.

Glycerol

None

MANUFACTURER DISCLAIMER: The information given herein is offered in good faith as accurate, but without guarantee. Conditions of the use and suitability of the product for particular uses are beyond our control. All risks of use of the product are therefore assumed by the user. Nothing is intended as a recommendation for uses which infringe valid patents or as extending license under valid patents. Appropriate warnings and safe handling procedures should be provided to handlers and users.