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Electrophoresis

Bis-AcrylaGel

$35.00$54.00

Quantity Discount when you buy 4 or more 1 Liter Units

Catalog Number: EC-820

  • Stabilized Bis-Acrylamide Solution
  • Deionized and Pre-filtered
  • Aldehyde Free and Acrylic Acid Free
  • Stabilized for a Two Year Shelf Life

Description

Quantity Discount when you buy 4 or more 1 Liter Units

Catalog Number: EC-820

  • Stabilized Bis-Acrylamide Solution
  • Deionized and Pre-filtered
  • Aldehyde Free and Acrylic Acid Free
  • Stabilized for a Two Year Shelf Life

Bis-AcrylaGel is a ready-to-use, 2% acrylamide solution in distilled/deionized water. Bis-AcrylaGel contains 20 grams of bis-acrylamide per liter of solution.

Bis-AcrylaGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20 oC).

Additional information

Weight 2.5 lbs
Dimensions 7 × 7 × 14 in

Protocol

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Calculate how much AcrylaGel and Bis-AcrylaGel you need to make your gels by using the formulas below. Bring up to the desired final volume with your usual buffers and distilled water.

Mix Gel Solution
Va = (A)(Vt)/30

Vb = (A)(C)(Vt)/200
Va = Volume of AcrylaGel to be used (ml),
Vb = Volume of Bis-AcrylaGel to be used (ml),
Vt = Total volume of gel casting solution desired (ml),
A = % acrylamide desired in gel,
C = % crosslinker desired

EXAMPLE: To make 100ml of a 10% acrylamide gel 2.7% crosslinked with bis, calculate the volume to be added as follows:
Va = (10) (100)/30 = 33.3ml AcrylaGel
Vb = (10) (2.7) (100)/200 = 13.5ml Bis-AcrylaGel

Add APS and Cast Gel
Add 1.0ml of 10% (w/v) FRESHLY PREPARED Ammonium Persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1ml of TEMED for every 100ml of gel casting solution. Swirl gently
to mix. Pour the solution into the gel casting cassette.

The gel should begin to set in 10-20 minutes.

Safety Overview

Safety Summary (see MSDS for complete information before using product):

EMERGENCY OVERVIEW – IMMEDIATE HAZARD

WARNING! HARMFUL IF SWALLOWED. MAY BE HARMFUL IF INHALED OR ABSORBED THROUGH SKIN. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. MAY AFFECT CENTRAL NERVOUS SYSTEM.

Full MSDS Protocols

Catalog Number: EC-820
DOWNLOAD MSDS PDF >