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SequaGel UreaGel 29:1 Denaturing Gel System

Price range: $136.00 through $221.00

Quantity discount when you buy 4 or more 2.2 liter kits!
Cataglog number: EC-829
  • Casts 29:1 denaturing gels for RNA or DNA analysis
  • Easily cast up to 20% gels
  • Certified nuclease-free
  • Consistently crystal clear gels
SKU: EC-829 Categories: , , ,

Description

Quantity discount when you buy 4 or more 2.2 liter kits!
Cataglog number: EC-829
  • Casts 29:1 denaturing gels for RNA or DNA analysis
  • Easily cast up to 20% gels
  • Certified nuclease-free
  • Consistently crystal clear gels
The newest addition to the SequaGel family, the UreaGel 29:1 System allows researchers to easily and safely produce denaturing gels of the popular 29:1 acrylamide:bis-acrylamide formula in concentrations up to 20% monomer. These gels are ideal for analysis of DNA or RNA. The UreaGel 29:1 System consists of UreaGel 29:1 Concentrate, UreaGel Buffer and UreaGel Diluent. UreaGel 29:1 Concentrate contains 241.7 g acrylamide, 8.3 g methylene:bisacrylamide per liter in 7.5M urea in a deionized aqueous solution. UreaGel Diluent consists of a deionized aqueous solution of 7.5M urea. UreaGel Buffer contains 0.89M Tris-Borate, 20mM EDTA and 7.5M urea at pH 8.3.
Storage: UreaGel 29:1 Concentrate, UreaGel Diluent, and UreaGel Buffer are stable for one year when stored tightly capped, in a dark area at room temperature (20 oC).

Additional information

Weight 5 lbs
Dimensions 14 × 7 × 18 in

Protocol

Procedure

Mix UreaGel System Components

Determine how much UreaGel 29:1 Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix.

For 100 ml Gel Casting Solution:

Vc = (Vt) (X)/25
Vb = 0.1 (Vt)
Vd = Vt – (Vc + Vb)

where:

Vc = UreaGel 29:1 Concentrate Volume
Vb = UreaGel Buffer Volume
Vd = UreaGel Diluent Volume
Vt = Total Casting Solution Volume
X = % Gel Desired

EXAMPLE:

To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows:
Vc =(100) (8)/25 = 32ml UreaGel 29:1 Concentrate
Vb = 0.1 (100) = 10ml UreaGel Buffer
Vd = 100 – (32 + 10) = 58ml UreaGel Diluent

Add Initiators and Cast Gel

Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.

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Safety Overview

Safety Summary (see MSDS for complete information before using product):

UreaGel 29:1 Concentrate
Appearance and Odor:
Clear colorless solution

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
WARNING! ACRYLAMIDE IS A NEUROTOXIN. HARMFUL IF SWALLOWED. sYMPTOMS OF EXPOSURE MAY INCLUDE NUMBNESS, TINGLING, SWEATING AND LOSS OF COORDINATION. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION.

EMERGENCY OVERVIEW – CHRONIC HAZARD WARNING
CHRONIC TOXICITY HAZARD. ACRYLAMIDE MAY CAUSE NERVOUS SYSTEM DAMAGE. ACRYLAMIDE CAUSED CANCER AND MALE REPRODUCTIVE DISORDERS IN LABORATORY ANIMAL TESTS. RISK DEPENDS ON DURATION AND LEVEL OF EXPOSURE.

UreaGel Buffer
Appearance and Odor
Clear colorless solution
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
Urea
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR INHALED.
Boric Acid
CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT. BORIC ACID IS HARMFUL IF SWALLOWED OR INHALED.
Tris-Base
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. HARMFUL IF SWALLOWED OR INHALED.

UreaGel Diluent
Appearance and Odor:
Clear, colorless solution

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
CAUSES IRRITATION TO SKIN, EYES, AND RESPIRATORY TRACT. UREA IS HARMFUL IF SWALLOWED OR INHALED.

Full SDS

UreaGel 29:1 Concentrate
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UreaGel Diluent
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UreaGel Buffer
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