Glass Free

  • Coats glass casting plates for easy release
  • Prevents gel from binding to the upper casting plate
Catalog number: EC-621
Glass Free from National Diagnostics coats glass casting plates for easy release of polyacrylamide gels. Glass Free prevents the cast gel from binding to the removable upper glass casting plate. Glass Free, used in conjunction with Glass Bond, guarantees whole, manageable electrophoretic gels time after time. Glass Free is reusable and has a shelf life of two years.
Storage: Glass Free is best stored tightly capped in a cool dry area.
Glass Free method of use:
  1. For preparing glass plates for gel casting, Glass Free is to be used only on the removable upper plate. Clean the glass with detergent, water, distilled water, and methanol. If the glass plate has previously been treated with Glass Bond, it is first necessary to soak the glass in 20% sodium hydroxide for 3-16 hours and then wash with water and distilled water.
  2. After the plate is thoroughly dry, immerse it in a dish of Glass Free for 5 minutes, making sure that all areas of the glass are well covered by the reagent. Remove any trapped air bubbles. Always work with Glass Free in a hood. Use latex, PVC or polyethylene gloves.
  3. Remove the glass plate from the Glass Free vessel and thoroughly rinse it with an organic solvent such as toluene, xylene, or mineral spirits.
  4. Rinse the solvent off with methanol. Lightly buff out the methanol with a paper towel. The plates are now ready for use. Store them in a plastic bag interleaved with paper.
  5. To reuse a plate that has previously been treated with Glass Free, wash the plate with water and distilled water and follow by a final rinse with methanol and buff.

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