Denhardt's Solution (50X)
- DNase and RNase free
- Made with 18 megOhm water
- 0.2 micron filtered
Catalog number: EC-915
Component of hybridization solutions for Northern and Southern blotting. Increases speed and sensitivity of hybridization reactions.
Product Catalog
Matrices
- AccuGel 19:1 (30%)
- AccuGel 19:1 (40%)
- AccuGel 29:1 (30%)
- AccuGel 29:1 (40%)
- AcrylaGel
- Bis-AcrylaGel
- ▼ For Proteins ▼
- ProtoGel (30%)
- ProtoGel (40%)
- ProtoGel Quick-Cast 12%
- ▼ For DNA and RNA ▼
- SequaGel XR Concentrate
- SequaGel - UreaGel Concentrate
- SequaGel - UreaGel 6
- SequaGel - UreaGel 8
- SequaGel - UreaGel System
- SequaGel UreaGel 29:1 Denaturing Gel System
- Sequagel Ureagel 29:1 Concentrate
- SequaGel XR
- SequaGel MD
- Sequagel MD SSCP Kit
- Sequagel MD Heteroduplex Kit
- ▼ Agarose ▼
- AquaPor LE GTAC
- AquaPor 3:1 GTAC
- AquaPor LM GTAC
- AquaPor ES GTAC
- AquaPor HR GTAC
Buffers
- ▼DNA/RNA Buffers▼
- TTE Glycerol Tolerant Buffer (20X)
- TBE Buffer (10X)
- TBE Buffer (5X)
- TAE Buffer (50X)
- SequaGel - UreaGel Buffer
- SequaGel - UreaGel Diluent
- SSC Buffer (20X)
- SSPE (20X)
- Denaturation Solution
- Neutralization Solution
- Sodium Acetate 3M Solution, pH 4.5
- Sodium Acetate 3M Solution, pH 5.2
- Sodium Acetate 3M Solution, pH 7.0
- Potassium Acetate, 1M Solution
- Potassium Acetate, 5M Solution
- TE Buffer (100X)
- ▼Protein Buffers▼
- ProtoBlot Rapid Western Blotting Buffer (10X)
- ProtoGel Resolving Buffer (4X)
- ProtoGel Stacking Buffer
- Tris-Glycine-SDS PAGE Buffer (10X)
- Tris-Tricine-SDS PAGE Buffer (10X)
- Tris-Glycine Electroblotting Buffer (10X)
- MES-SDS Running Buffer (20X)
- MESA RNA Electrophoresis Buffer (10X)
- MOPS-SDS Running Buffer (20X)
- PBS (10X) Phosphate Buffered Saline
- TBS (10X)
- TBST (10X)
- ProtoLift Western Stripping Buffer
Detection Systems
Sample Preparation and Markers
Ultra Pure Reagents
- Acrylamide - ULTRA PURE
- BIS - ULTRA PURE
- 2-Mercaptoethanol - ULTRA PURE
- Ammonium Persulfate - ULTRA PURE
- Boric Acid - ULTRA PURE
- Bromocresol Green
- Bromophenol Blue
- Coomassie Blue G-250
- DATD - ULTRA PURE
- DEPC Treated Water
- Dextran Sulfate - ULTRA PURE
- Denhardt's Solution (50X)
- Dithiothreitol - ULTRA PURE
- EDTA - ULTRA PURE
- EDTA, 0.5M Solution
- Formamide - ULTRA PURE
- Glass Bond
- Glass Free
- Glycerol - ULTRA PURE
- Glycine - ULTRA PURE
- Ion Exchange Resin (mixed bed)
- Methylene Blue
- Potassium Chloride
- Riboflavin - ULTRA PURE
- SDS - ULTRA PURE
- SDS Solution (20%)
- Sodium Chloride, 1M Solution
- Sodium Chloride, 0.9 Percent Solution
- TEMED - ULTRA PURE
- Tricine - ULTRA PURE
- Tris - ULTRA PURE
- Tris-HCl, pH 7.2
- Tris-HCl, pH 7.4
- Tris-HCl, pH 7.6
- Tween-20 - ULTRA PURE
- Urea - ULTRA PURE
- Xylene Cyanole FF
Articles
Fundamentals of Electrophoresis
- Biological Macromolecules
- Biological Macromolecules - Proteins
- The Mechanical and Electrical Dynamics of Gel Electrophoresis - Intro and Sample Mobility
- The Mechanical and Electrical Dynamics of Gel Electrophoresis - Ohm's Law
- The Mechanical and Electrical Dynamics of Gel Electrophoresis -- Electrophoresis System Dynamics
- The Electrophoresis Matrix
- The Polyacrylamide Matrix
- The Polyacrylamide Matrix-Buffer Strength
- The Agarose Matrix
- Electrophoresis Buffers--The Henderson-Hasselbalch Equation
- Electrophoresis Buffers-Choosing the Right Buffer
- Homogeneous Buffer Systems
- Multiphasic Buffer Systems
- Buffer Additives-Hydrogen Bonding Agents
- Buffer Additives-Surfactants
- Buffer Additives-Reducing Agents
- Horizontal and Vertical Gel Systems - The Horizontal Gel System
- Horizontal and Vertical Gel Systems - Vertical Tube Gels
- Horizontal and Vertical Gel Systems - The Vertical Slab Gel System
Electrophoresis of DNA and RNA
- Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA
- Sample Prep for Denaturing PAGE of DNA
- Preparing Denaturing DNA & RNA Gels
- Run Conditions in Denaturing PAGE
- Using PAGE to Determine Nucleic Acid Molecular Weight
- Manual Sequencing
- Maxam & Gilbert Sequencing
- Sanger Sequencing
- Pouring Sequencing Gels
- Automated Sequencers
- Differential Display
- RNA Mapping
- S1 Mapping
- Ribonuclease Protection
- Primer Extension
- Analysis of DNA/Protein Interactions
- Methylation & Uracil Interference Assays
- Native PAGE of DNA
- Sample Preparation for Native PAGE of DNA
- Gel Preparation for Native PAGE of DNA
- PCR Analysis: An Examination
- PCR Analysis: Yield and Kinetics
- Gel Electrophoresis of PCR Products
- Mobility Shift Assay
- DNA/RNA Purification from PAGE Gels
- Conformational Analysis
- Agarose Gel Electrophoresis of DNA and RNA - An Introduction
- Heteroduplex Analysis
- SSCP Analysis
- Preparation of Agarose Gels
- Agarose Gel Electrophoresis of DNA and RNA - Uses and Variations
- Restriction Digest Mapping
- In Gel Enzyme Reactions
- DNA/RNA Purification from Agarose Gels - Electroelution
- PFGE and FIGE
- RNA Electrophoresis
- Preparation of Denaturing Agarose Gels
- Gel Electrophoresis of RNA & Post Electrophoretic Analysis
Electrophoresis of Proteins
- Denaturing Protein Electrophoresis: SDS-PAGE
- Sample Preparation for SDS-PAGE
- Gel Preparation for SDS-PAGE
- Casting Gradient Gels
- Measuring Molecular Weight with SDS-PAGE
- Peptide Mapping
- Native Protein Electrophoresis
- Gel Preparation for Native Protein Electrophoresis
- Activity Stains
- Immuno-Electrophoresis / Immuno-Diffusion
- Isoelectric Focusing
- Isotachophoresis
Post Electrophoretic Analysis
- UV Shadowing
- Post-Electrophoretic Visualization with Nuclistain
- Ethidium Bromide Staining
- Silver Staining DNA Gels
- An Overview of Northern and Southern Blotting
- Northern Blotting
- Southern Blotting
- Alkaline Blotting
- Autoradiography
- Autoradiographic Enhancement with Autofluor
- Protein Fixation on Gels
- Staining Protein Gels with Coomassie Blue
- Silver Staining Protein Gels
- Guide Strip Technique
- Staining Proteins Immobilized on Membranes
- Mechanism of Immunostaining
- Immunostaining with Horseradish Peroxidase
- Immunostaining with Alkaline Phosphatase
- Enzyme Linked Immunosorbent Assay (ELISA)
- Overview of Western Blotting
- Method for Western Blotting
Protocols
Product Protocols
- Accugel
- Acrylagel
- Aquapor Agaroses
- Autofluor
- NucliStain
- ProtoBlue Safe
- SequaGel UreaGel System
- Sterling Silver Stain l
- Protein Precipitation Kit
- ProtoBlock System
- ProtoGel (30%)
- ProtoGel (40%)
- ProtoGel Quick Cast
- ProtoGel Sample Prep Kit
- ProtoGlow ECL
- SequaGel MD
- SequaGel UreaGel 29:1 Denaturing Gel System
- Sequagel XR
- UreaGel 6 and 8
Technique Protocols
SDS
A-F
- AccuGel 19:1 (30%)
- AccuGel 19:1 (40%)
- AccuGel 29:1 (30%)
- AccuGel 29:1 (40%)
- AcrylaGel
- Acrylamide
- Ammonium Persulfate
- AquaPor 3:1
- AquaPor ES
- AquaPor HR
- AquaPor LE
- AquaPor LM
- Bis-Acrylagel
- Boric Acid
- DATD
- Denaturation Solution
- Dextran Sulfate
- Dithiothreitol (DTT) Safety Data
- EDTA 0.5M Safety Data
- EDTA
- Formamide
G-N
- Glass Bond
- Glass Free
- Glycerol
- Glycine
- HRPL Kit
- Insite Buffer Concentrate
- Insite Dye Concentrate
- Ion Exchange Resin
- 2-Mercaptoethanol
- MES-SDS Running Buffer (20X) Safety Data
- MESA RNA Electrophoresis Buffer Safety Data
- Methylene Bis-Acrylamide
- MOPS-SDS Running Buffer (20X) Safety Data
- ND Protein Precipitation Kit - Reagent A
- ND Protein Precipitation Kit - Reagent B
- Neutralization Solution
- Nuclistain Safety Data
O-S
- PBS (10X) Phosphate Buffered Saline Safety Data
- Protein Loading Buffer Blue (2x)
- 5X Protein Loading Buffer
- ProtoBlock System Reagent A Safety Data
- ProtoBlock System Reagent B Safety Data
- ProtoBlue Safe Safety Data
- ProtoGel (30%)
- ProtoGel (40%)
- ProtoGel Quick-Cast 12%
- ProtoGel Sample Prep Kit (A)
- ProtoGel Sample Prep Kit (B)
- ProtoGlow ECL Reagent A Safety Data
- ProtoGlow ECL Reagent B Safety Data
- ProtoGel Stacking Buffer
- ProtoGel Buffer
- Protomarkers
- Protometrics
- Riboflavin
- SSC Buffer
- SSCP Stop Solution
- SDS Solution 20%
- SequaGel MD Monomer
- Sequagel XR
- SequaGel XR Concentrate
- Sodium Dodecyl Sulfate
- Sterling Silver Stain
- Sterling Silver Stain Part A
- Sterling Silver Stain Part B
- Potassium Chloride 1M Safety Data
- ProtoBlot Rapid Western Blotting Buffer (10X) Safety Data
- ProtoBlot Rapid Western Transfer Buffer (10X) Safety Data
- ProtoLift Western Stripping Buffer Safety Data
- ProtoStain Blue Safety Data
- Sodium Chloride 0.9%
- Sodium Chloride 1M
T-Z
- TAE Buffer
- TBE Buffer (10X)
- TBE Buffer (5X)
- TBS (10X) Safety Data
- TBST (10X) Safety Data
- TE Buffer
- TEMED
- TWEEN-20
- TTE Buffer
- Tricine
- Triple Dye Loading Buffer
- Tris
- Tris Glycine Electroblotting Buffer
- Tris Tricine SDS PAGE Buffer
- Tris-Glycine-SDS PAGE Buffer
- Urea
- UreaGel 29:1 Concentrate Safety Data
- UreaGel Buffer
- UreaGel 6
- UreaGel 8
- UreaGel Complete Buffer
- UreaGel Concentrate
- UreaGel Diluent
- Tris-HCL pH 7.6 Safety Data
- Tris-HCl pH 7.2
- Tris-HCl pH 7.4 Safety Data
- UreaGel Complete Buffer
- UreaGel Loading Buffer
- DEPC Treated Water Safety Data
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