Electrophoresis
AquaPor LM GTAC Agarose
Price range: $165.00 through $485.00
- Low Melting Temperature
- DNase and RNase free
- Exceptional Gel Strength for a Low Melt Agarose
- Fastest Dissolving
Description
- Low Melting Temperature
- DNase and RNase free
- Exceptional Gel Strength for a Low Melt Agarose
- Fastest Dissolving
AquaPor LM GTAC is a low melting temperature agarose for both large and small DNA fragments. For large fragments up to 25 kb, a 1% AquaPor LM solution forms a gel strong enough to be handled without fracturing. For separation of smaller fragments from 20 bp to 1,000 bp, AquaPor LM possesses low viscosity so that 3% or 4% gels can be made. DNA fragments separated in AquaPor LM may be immediately used for enzymatic manipulation in the presence of the remelted agarose. Ultra-fine particle size ensures fastest dissolution. AquaPor LM is certified to be DNase, RNase, and protease free. AquaPor LM is certified for in-gel PCR (re)amplification and in-gel ligation.
| Melt Temp (oC) | Gel Strength (g/cm2) | Gel Temp (oC) |
| ≤ 65 @ 1.0% | ≥ 450 @ 1.0% | ≤ 27 @ 1.0% |
Additional information
| Weight | 0.2 lbs |
|---|---|
| Dimensions | 6 × 3 × 3 in |
Protocol
DISSOLVING AQUAPOR AGAROSES
- Add room temperature buffer to a flask that is 2.5 – 4 times the volume of gel solution. Add a teflon-coated stir bar.
- Add AquaPor powder while stirring vigorously so the agarose is dispersed uniformly. Stir for 2 minutes to hydrate the agarose.
- Tare the flask and solution.
- Place in a microwave oven and heat at 100% power using 20 – 60 second intervals. Swirl gently between intervals to resuspend the agarose.
- Continue the cycle of heating and swirling until the agarose is completely dissolved (no visible particles are present).
- Add distilled water to obtain the initial weight and mix.
- Cool the solution to 50 – 60°C before pouring the gel.
* Chill the gel for 30 minutes prior to comb removal when using AquaPor LM, HR, and low (<1%) concentration of AquaPor LE and ES.
Safety Overview
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
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