News

October 4, 2013

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

5X Protein Loading Buffer

  • Load more sample per well
  • Lithium Dodecyl Sulfate give improved resolution

 

Catalog number: EC-887

5X Protein Loading Buffer is a reducing sample buffer for SDS-polyacrylamide gel electrophoresis (SDS-PAGE). 5X Protein Loading Buffer contains 1.0M TrisHCl (pH 8.5), 8% (w/v) lithium dodecyl sulfate, 40% (v/w) glycerol, 2mM EDTA, 0.5M DTT and tracking dye in distilled/deionized water.

More concentrated loading buffer means less sample dilution, with more protein loaded per well.  The use of lithium dodecyl sulfate, in place of the more common sodium salt, give the best possible resolution.