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Electrophoresis

SequaGel UreaGel 29:1 Concentrate

$68.00$125.00

Quantity Discount when you buy 4 or more 1 Liter Units
Catalog Number: EC-828

UreaGel 29:1 Concentrate is one of three components of the UreaGel 29:1 System. The UreaGel 29:1 System consists of UreaGel 29:1 Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare 29:1 gels of varying percentages. With the UreaGel 29:1 System, the researcher readily prepares any commonly used gel formulation up to 20% monomer (29:1 acrylamide: bisacrylamide). Liter bottles of UreaGel Concentrate contain 241.6 grams of acrylamide, 8.3 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution.

Description

Quantity Discount when you buy 4 or more 1 Liter Units
Catalog Number: EC-828

UreaGel 29:1 Concentrate is one of three components of the UreaGel 29:1 System. The UreaGel 29:1 System consists of UreaGel 29:1 Concentrate, UreaGel Diluent, and UreaGel Buffer. This system provides a convenient, dependable means for researchers to prepare 29:1 gels of varying percentages. With the UreaGel 29:1 System, the researcher readily prepares any commonly used gel formulation up to 20% monomer (29:1 acrylamide: bisacrylamide). Liter bottles of UreaGel Concentrate contain 241.6 grams of acrylamide, 8.3 grams of methylene bisacrylamide, and 7.5M urea in a deionized aqueous solution.

Additional information

Weight 2.5 lbs
Dimensions 7 × 7 × 14 in

Protocol

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Mix UreaGel System Components:
Determine how much UreaGel 29:1 Concentrate, Diluent, and Buffer you need to make your gels using the formulas below. Combine the necessary components in an Erlenmeyer flask. Swirl gently to mix.

For 100 ml Gel Casting Solution:

Vc = (Vt) (X)/25
Vb = 0.1 (Vt)
Vd = Vt – (Vc + Vb)

where:

Vc = UreaGel 29:1 Concentrate Volume
Vb = UreaGel Buffer Volume
Vd = UreaGel Diluent Volume
Vt = Total Casting Solution Volume
X = % Gel Desired

EXAMPLE:

To make 100ml of an 8% gel, calculate the UreaGel solution volume to be added as follows:
Vc =(100) (8)/25 = 32ml UreaGel 29:1 Concentrate
Vb = 0.1 (100) = 10ml UreaGel Buffer
Vd = 100 – (32 + 10) = 58ml UreaGel Diluent

Add Initiators and Cast Gel:
Add 40 microliters of TEMED for every 100 ml of gel casting solution. Swirl gently to mix. Add 0.8 ml of FRESHLY PREPARED 10% ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Cast the gel. Insert the comb and allow to polymerize one to two hours.

 

Catalog Number:
EC-829
EC-828
EC-840
EC-835

lution

Va = (A)(Vt)/30

Vb = (A)(C)(Vt)/200
Va = Volume of AcrylaGel to be used (ml),
Vb = Volume of Bis-AcrylaGel to be used (ml),
Vt = Total volume of gel casting solution desired (ml),
A = % acrylamide desired in gel,
C = % crosslinker desired

EXAMPLE: To make 100ml of a 10% acrylamide gel 2.7% crosslinked with bis, calculate the volume to be added as follows:
Va = (10) (100)/30 = 33.3ml AcrylaGel
Vb = (10) (2.7) (100)/200 = 13.5ml Bis-AcrylaGel

Add APS and Cast Gel
Add 1.0ml of 10% (w/v) FRESHLY PREPARED Ammonium Persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1ml of TEMED for every 100ml of gel casting solution. Swirl gently
to mix. Pour the solution into the gel casting cassette.

The gel should begin to set in 10-20 minutes.

Full MSDS Protocols

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