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Electrophoresis

SequaGel MD SSCP Kit

$170.00

Quantity Discount when you buy 4 or more


Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.

Catalog number: EC-846
Size: 1 Kit

Point Mutation Analysis

Matrix and Loading Buffer Optimized for SSCP

Description

Quantity Discount when you buy 4 or more


Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.

Catalog number: EC-846
Size: 1 Kit

Point Mutation Analysis

Matrix and Loading Buffer Optimized for SSCP

SequaGel MD SSCP Kit permits minor mutational differences in DNA sequences to be detected as a high-resolution relative mobility (Rf) shift. SequaGel MD is a proprietary formulation, supplied as a 2X stock, designed to resolve sequence-related differences by SSCP (Single Strand Conformational Polymorphism).

DNA mutations, or sequence modifications, are readily associated with specific disease states. Since a variation of a single nucleotide in a sequence may indicate a significant genetic anomaly, an extremely sensitive method to analyze these mutations is necessary.

SequaGel MD is designed to run SSCP samples in the 100-300 nucleotide range at a 0.5X concentration. Small fragments may be better resolved by using higher concentration (0.75X) gels. Larger fragments may be better resolved by running lower concentration (0.4X) gels.

The kit contains 200ml of Sequagel MD Monomer and SSCP Stop Solution.

P samples in the 100-300 nucleotide range at a 0.5X concentration. Small fragments may be better resolved by using higher concentration (0.75X) gels. Larger fragments may be better resolved by running lower concentration (0.4X) gels. SequaGel MD is designed to run heteroduplex analysis on DNA fragments up to 900 bases at a 1X concentration.

By preventing band inversions, SequaGel XR bands are sharp and true. SequaGel XR provides more information by allowing greater separation between bands near the top of the gel. This effect, similar to a wedge gel, is achieved with standard spacers.

The quality and consistency of SequaGel XR is evidenced by the uniform background obtained upon drying. Band edges are sharp. Even without fixing, the gel will dry without cracking or crazing. The result is a finely-rendered, highly detailed DNA gel.

SequaGel XR Concentrate is manufactured as a 50% stock solution. SequaGel XR is also shipped as a two-component system consisting of the SequaGel XR Monomer Solution and SequaGel Complete Buffer. SequaGel Complete Buffer Solution contains TBE and TEMED. No dilutions are necessary in this easy-to-use system. The researcher simply combines the two solutions, adds ammonium persulfate, and casts the gel.

Storage: SequaGel XR Concentrate is stable for one year when stored tightly capped, in a dark area at room temperature (20°C).

Additional information

Weight 2 lbs
Dimensions 7 × 7 × 11 in

Safety Overview

Safety Summary (see MSDS for complete information before using product):

Appearance and Odor:
Clear colorless solution

May cause cancer. May cause heritable genetic damage. Also toxic in contact with skin and if swallowed. Danger of serious damage to health by prolonged exposure through inhalation, in contact with skin or if swallowed.
Avoid exposure, obtain special instructions before use. In case of accident or if you feel ill, seek medical advice immediately (show the label where possible).

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
WARNING! ACRYLAMDE IS A NEUROTOXIN. HARMFUL IF SWALLOWED. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION.

Full MSDS Protocols

Catalog Number: EC-845

DOWNLOAD MSDS PDF >