Electrophoresis - Color Icons2

Electrophoresis

ND Protein Precipitation Kit

$39.00

Catalog number: EC-888
Size: 1 Kit

  • Recovery of All Proteins from Complex Mixtures
  • Precipitates as Little as 100ng of BSA at 0.25µg/ml
  • Milder than TCA
  • More Reliable than Acetonitrile or Ammonium Sulfate

Description

Catalog number: EC-888
Size: 1 Kit

  • Recovery of All Proteins from Complex Mixtures
  • Precipitates as Little as 100ng of BSA at 0.25µg/ml
  • Milder than TCA
  • More Reliable than Acetonitrile or Ammonium Sulfate

The ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of any procedure, allowing the high yield collection of all proteins in solution, precipitating even the most dilute proteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by other methods.

Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone among all methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation.


High Yield Recovery

10ug BSA in 1ml vol and 50 ng of BSA in 200ul were prepared from stock and precipitated following kit instructions. 10ug and 50ng of BSA in 5 ul were prepared as controls. After running on a 12% gel, lanes 2 and 3 show control and recovered 10ug BSA. Lanes 5 and 6 show control and recovered 50ng BSA. Densitometry of 10ug BSA showed 91.9% recovery

.

Simplicity of Method

Reagent A and Reagent B form a coprecipitate with the protein.

After a simple wash, the protein pellet is ready to be redissolved in desired buffer.


Additional information

Weight 1 lbs
Dimensions 8 × 3 × 10 in

Protocol

Download Full Protocol PDF >

Procedure:

  1. Add 1/20 volume Reagent A to sample in a centrifuge tube and mix well.
  2. Add 1/10 volume Reagent B to sample.
  3. Allow to precipitate for 20 minutes at room temperature. Precipitate is comprised of Reagent A:B complex along with trapped protein molecules.
  4. Collect precipitate by centrifugation and remove supernatant. The pellet will be large.
  5. Completely disperse pellet in acetone to dissolve away precipitated A:B complex. The solution should appear clear to cloudy, depending on protein concentration, with no visible clumps. Undispersed clumps will trap impurities which will be carried over into the final isolate.
  6. Collect proteins by centrifugation.
  7. To remove salts and surfactants, wash pellet with acetone, acetonitrile or 70% ethanol. This step may be repeated if desired for heavily contaminated samples, or for downstream applications requiring the highest purity proteins. Collect proteins by brief centrifugation if necessary.
  8. Redissolve pellet in desired buffer.

Safety Overview

Safety Summary (see MSDS for complete information before using product):

Catalog Number: EC-888A

Appearance and Odor
Clear colorless aqueous Solution
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
May be harmful if swallowed. May cause severe eye irritation and possible injury. Causes skin and respiratory tract irritation.


Catalog Number: EC-888B

Safety Summary (see MSDS for complete information before using product):

Appearance and Odor
Aqueous Solution
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
WARNING! HARMFUL IF SWALLOWED OR INHALED. CAUSES IRRITATION TO SKIN, EYES AND RESPIRATORY TRACT.

Full MSDS Protocols

ND Protein Precipitation Kit – Reagent A
Download Full MSDS Protocols >

ND Protein Precipitation Kit – Reagent B
Download Full MSDS Protocols >