
Electrophoresis
Autofluor
$299.00
Quantity discount when you buy four or more bottles!Catalog Number: LS-315Size: 1 liter
- High-resolution autoradiographic image intensifier
- Rapid enhancement of low energy beta-emitters
- For polyacrylamide gels, paper chromatography, and TLC plates
- Water-based, odorless, contains no DMSO
Description
Catalog Number: LS-315
Size: 1 liter
- High-resolution autoradiographic image intensifier
- Rapid enhancement of low energy beta-emitters
- For polyacrylamide gels, paper chromatography, and TLC plates
- Water-based, odorless, contains no DMSO
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In the figure to the left, the gel on the left (7%, 1mm) was dehydrated in DMSO (dimethylsulfoxide) for one hour, then impregnated in PPO-DMSO for one hour and precipitated and dried. The gel on the right was impregnated with Autofluor for one hour and dried. Both gels were exposed for 24 hours at -76°C on Kodak XR-5 X-OMat film. The single tritiated band contains 5000 dpm. Note the higher degree of resolution and band discrimination with Autofluor vs PPO-DMSO.The Autofluor procedure is the shortest and easiest procedure yet developed for enhancement and visualization of beta-emitters. In an independent test comparing eight different fluorographic methods for the detection of 35S-labeled proteins in polyacrylamide gels, Autofluor was the most effective. With Autofluor, the dpm/mm2 required to half-saturate the x-ray film was 1/8 that required by autoradiography alone.
Storage: Autofluor has a shelf life of at least one year stored at room temperature, out of direct sunlight. Keep from freezing. At temperatures less than 20°C precipitation of water soluble phosphors may occur. Warming to approximately 30°C will redissolve the phosphors.