Electrophoresis
AquaPor 3:1 GTAC Agarose
Price range: $119.00 through $315.00
Catalog Number: EC-206
Quantity discount when you buy 4 or more 100 g units!
- Small Fragment DNA and RNA
- RT-PCR
- Ultra-Fine particle size for fastest dissolution
Description
Catalog Number: EC-206
Quantity discount when you buy 4 or more 100 g units!
- Small Fragment DNA and RNA
- RT-PCR
- Ultra-Fine particle size for fastest dissolution
AquaPor 3:1 GTAC is a molecular biology grade agarose specifically manufactured to yield strong gels for fine resolution of small DNA. It makes an excellent matrix for the resolution of small fragment DNA and RNA. AquaPor 3:1 may be used confidently for Southern and Northern blotting, as well as DNase footprinting and RT-PCR. AquaPor 3:1 is GTAC certified to be DNase, RNase, and protease-free.
| EEO (-mr) | Gel Strength (g/cm2) | Gel Temp (°C) |
| ≤ 0.07 | ≥ 1200 @ 1.0% | ≤ 40 @ 4.0% |
| Melt Temp (°C) | Sulfate (%) | DNase, RNase, Protease |
| ≤ 92 @ 4.0% | ≤ 0.20 | >None |
Additional information
| Weight | 0.2 lbs |
|---|---|
| Dimensions | 6 × 3 × 3 in |
Protocol
DISSOLVING AQUAPOR AGAROSES
- Add room temperature buffer to a flask that is 2.5 – 4 times the volume of gel solution. Add a teflon-coated stir bar.
- Add AquaPor powder while stirring vigorously so the agarose is dispersed uniformly. Stir for 2 minutes to hydrate the agarose.
- Tare the flask and solution.
- Place in a microwave oven and heat at 100% power using 20 – 60 second intervals. Swirl gently between intervals to resuspend the agarose.
- Continue the cycle of heating and swirling until the agarose is completely dissolved (no visible particles are present).
- Add distilled water to obtain the initial weight and mix.
- Cool the solution to 50 – 60°C before pouring the gel.
* Chill the gel for 30 minutes prior to comb removal when using AquaPor LM, HR, and low (<1%) concentration of AquaPor LE and ES.
Safety Overview
Appearance and Odor
White powder
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
AS PART OF GOOD INDUSTRIAL AND PERSONAL HYGIENE AND SAFETY PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.
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