Electrophoresis - Color Icons2

Electrophoresis

AcrylaGel

$66.00$118.00

Quantity Discount when you buy 4 or more 1 Liter Units

Catalog Number: EC-810

  • Stabilized Acrylamide Solution
  • Deionized and Pre-filtered
  • Aldehyde and Acrylic Acid-Free
  • Certified RNase and DNase Free
  • Stabilized for a Two Year Shelf Life

Description

Quantity Discount when you buy 4 or more 1 Liter Units

Catalog Number: EC-810

  • Stabilized Acrylamide Solution
  • Deionized and Pre-filtered
  • Aldehyde and Acrylic Acid-Free
  • Certified RNase and DNase Free
  • Stabilized for a Two Year Shelf Life

AcrylaGel is a ready-to-use, 30% acrylamide solution in distilled/deionized water. AcrylaGel contains 300 grams of acrylamide per liter of solution. Because acrylamide in its crystalline form is subject to self-polymerization, the purity and consistency of polymerized AcrylaGel is superior to that obtainable with powdered acrylamide.

AcrylaGel can be crosslinked with Bis-AcrylaGel, a ready-to-use, 2% solution of methylene bisacrylamide with the same advantages as the AcrylaGel acrylamide solution. However, any powdered crosslinking reagent can be used with AcrylaGel.

AcrylaGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20ºC).

Additional information

Weight 4.8 lbs
Dimensions 7 × 7 × 14 in

Protocol

Download Full Protocol PDF >

Calculate how much AcrylaGel and Bis-AcrylaGel you need to make your gels by using the formulas below. Bring up to the desired final volume with your usual buffers and distilled water.

Mix Gel Solution
Va = (A)(Vt)/30

Vb = (A)(C)(Vt)/200
Va = Volume of AcrylaGel to be used (ml),
Vb = Volume of Bis-AcrylaGel to be used (ml),
Vt = Total volume of gel casting solution desired (ml),
A = % acrylamide desired in gel,
C = % crosslinker desired

EXAMPLE: To make 100ml of a 10% acrylamide gel 2.7% crosslinked with bis, calculate the volume to be added as follows:
Va = (10) (100)/30 = 33.3ml AcrylaGel
Vb = (10) (2.7) (100)/200 = 13.5ml Bis-AcrylaGel

Add APS and Cast Gel
Add 1.0ml of 10% (w/v) FRESHLY PREPARED Ammonium Persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1ml of TEMED for every 100ml of gel casting solution. Swirl gently
to mix. Pour the solution into the gel casting cassette.

The gel should begin to set in 10-20 minutes.

Safety Overview

Safety Summary (see MSDS for complete information before using product):

EMERGENCY OVERVIEW – IMMEDIATE HAZARD

WARNING!  NEUROTOXIN.  HARMFUL IF SWALLOWED, INHALED OR ABSORBED THROUGH SKIN.  SYMPTOMS OF EXPOSURE MAY INCLUDE NUMBNESS, SWEATING OR ALTERED GAIT. MAY CAUSE ALLERGIC SKIN REACTION. MAY CAUSE EYE IRRITATION. POLYMERIZATION MAY OCCUR FROM EXCESSIVE HEAT OR CONTAMINATION. COMBUSTIBLE SOLID

Full MSDS Protocols

Catalog Number: EC-810
DOWNLOAD MSDS PDF >