Electrophoresis - Color Icons2

Electrophoresis

ProtoBlot Rapid Western Blotting Buffer (10X)

$49.00

Catalog number: EC-878
Size: 1 Liter
  • Blot in half the time versus conventional electroblotting buffers
  • Very high-efficiency transfer
  • Optimal pH; less harsh on proteins

Description

Catalog number: EC-878
Size: 1 Liter
  • Blot in half the time versus conventional electroblotting buffers
  • Very high-efficiency transfer
  • Optimal pH; less harsh on proteins

ProtoBlot Rapid Western Blotting Buffer is a relatively low conductivity buffer designed to replace tris/glycine (Towbin) buffer in wet (tank) transfer applications. It allows protein transfer from gels to nitrocellulose or PVDF at relatively higher voltages.

The speed of transfer of a protein from the gel to the membrane during a western blot is proportional to the electric field strength. It is directly proportional to the voltage applied and inversely proportional to the distance between the electrodes and is expressed in V/cm.  The amount of heat produced during the transfer is a function of the current flowing through the system. A lower conductivity buffer like ProtoBlot can be run at a higher voltage producing less heat because the current conducted will be lower. This allows for a shorter run time and more efficient transfer.

Key notes:

  • For use the 10X buffer is diluted to 1X and ethanol or methanol is added to 10%-20%.
  • The pH of the buffer at a 1X dilution is 8.4–8.5 which is the ideal pH for maximum transfer of most proteins.
  • The transfer can be done with or without an ice pack or cooling system.
  • Optimum transfer times will vary and have to be determined empirically, but a suggested starting point is 300V for 20 minutes.

Additional information

Weight 4.6 lbs
Dimensions 7 × 7 × 14 in

Safety Overview

Safety Summary (see SDS for complete information before using product):

Appearance and odor
Odorless, colorless solution.

EMERGENCY OVERVIEW – IMMEDIATE HAZARD
None

Full SDS

View full SDS >

Download full SDS >