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October 4, 2013

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

ND Protein Precipitation Kit

  • Recovery of All Proteins from Complex Mixtures
  • Precipitates as Little as 100ng of BSA at 0.25µg/ml
  • Milder than TCA
  • More Reliable than Acetonitrile or Ammonium Sulfate
Catalog number: EC-888
The ND Protein Precipitation Kit is mild on samples and easy to use. It casts the finest net of any procedure, allowing the high yield collection of all proteins in solution, precipitating even the most dilute proteins. The ND Protein Precipitation Kit allows collection of proteins that would be missed by other methods.
Ideal for both routine and difficult work, the ND Protein Precipitation Kit stands alone among all methods. It offers the best combination of mildness, simplicity and effectiveness in protein precipitation.
 


High Yield Recovery

10ug BSA in 1ml vol and 50 ng of BSA in 200ul were prepared from stock and precipitated following kit instructions. 10ug and 50ng of BSA in 5 ul were prepared as controls. After running on a 12% gel, lanes 2 and 3 show control and recovered 10ug BSA. Lanes 5 and 6 show control and recovered 50ng BSA. Densitometry of 10ug BSA showed 91.9% recovery.



Simplicity of Method

Reagent A and Reagent B form a coprecipitate with the protein.

After a simple wash, the protein pellet is ready to be redissolved in desired buffer.


 

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