Electrophoresis
AcrylaGel
Price range: $79.50 through $142.00
Catalog Number: EC-810
- Stabilized Acrylamide Solution
- Deionized and Pre-filtered
- Aldehyde and Acrylic Acid-Free
- Certified RNase and DNase Free
- Stabilized for a Two Year Shelf Life
Description
Catalog Number: EC-810
- Stabilized Acrylamide Solution
- Deionized and Pre-filtered
- Aldehyde and Acrylic Acid-Free
- Certified RNase and DNase Free
- Stabilized for a Two Year Shelf Life
AcrylaGel is a ready-to-use, 30% acrylamide solution in distilled/deionized water. AcrylaGel contains 300 grams of acrylamide per liter of solution. Acrylamide in its crystalline form is subject to self-polymerization, and as a result the purity and consistency of polymerized AcrylaGel is superior to that obtainable with powdered acrylamide.
AcrylaGel can be crosslinked with Bis-AcrylaGel, a ready-to-use, 2% solution of methylene bisacrylamide with the same advantages as the AcrylaGel acrylamide solution. However, any powdered crosslinking reagent can be used with AcrylaGel.
AcrylaGel is stable for 24 months when stored tightly capped in a dark area at room temperature (20ºC).
Additional information
| Weight | 4.8 lbs |
|---|---|
| Dimensions | 7 × 7 × 14 in |
Protocol
Procedure
Mix Gel Solution
Calculate how much AcrylaGel and Bis-AcrylaGel you need to make your gels by using the formulas below. Bring up to the desired final volume with your usual buffers and distilled water.
Va = (A)(Vt)/30
Vb = (A)(C)(Vt)/200
where,
Va = Volume of AcrylaGel to be used (ml),
Vb = Volume of Bis-AcrylaGel to be used (ml),
Vt = Total volume of gel casting solution desired (ml),
A = % acrylamide desired in gel,
C = % crosslinker desired
EXAMPLE: To make 100ml of a 10% acrylamide gel 2.7% crosslinked with bis, calculate the volume to be added as follows:
Va = (10) (100)/30 = 33.3ml AcrylaGel
Vb = (10) (2.7) (100)/200 = 13.5ml Bis-AcrylaGel
Add APS and Cast Gel
Add 1.0ml of 10% (w/v) FRESHLY PREPARED ammonium persulfate for every 100ml of gel casting solution. Swirl gently to mix. Add 0.1ml of TEMED for every 100ml of gel casting solution. Swirl gently to mix. Pour the solution into the gel casting cassette.
The gel should begin to set in 10-20 minutes.
Safety Overview
Safety Summary (see MSDS for complete information before using product):
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
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