Catalog number: EC-810

AcrylaGel Protocol

Calculate how much AcrylaGel and Bis-AcrylaGel you need to make your gels by using the formulas below. Bring up to the desired final volume with your usual buffers and distilled water.

Mix Gel Solution
Va = (A)(Vt)/30

Vb = (A)(C)(Vt)/200
Va = Volume of AcrylaGel to be used (ml),
Vb = Volume of Bis-AcrylaGel to be used (ml),
Vt = Total volume of gel casting solution desired (ml),
A = % acrylamide desired in gel,
C = % crosslinker desired


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