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Electrophoresis Articles

Autoradiography

By National Diagnostics | September 14, 2011 | Comments Off on Autoradiography

Autoradiography is the use of X-ray (or occasionally photographic) film to detect radioactive materials. It produces a permanent record of the positions and relative intensities of radiolabeled bands in a gel or blot. Typically, biomolecules…

Alkaline Blotting

By National Diagnostics | September 14, 2011 | Comments Off on Alkaline Blotting

One example of one might attempt alkaline blotting is listed here.

Southern Blotting

By National Diagnostics | September 14, 2011 | Comments Off on Southern Blotting

One possible procedure for Southern blotting can be found here.

Northern Blotting

By National Diagnostics | September 14, 2011 | Comments Off on Northern Blotting

The Procedure ELECTROPHORETIC SEPARATION RNA samples should be free of contaminating particles and DNA, with an A260/A280 ratio of 1.9-2.0. For highly expressed genes, load 10µg total of RNA. For trace expression detection, load 10µg…

An Overview of Northern and Southern Blotting

By National Diagnostics | September 12, 2011 | Comments Off on An Overview of Northern and Southern Blotting

A DNA or RNA probe will selectively hybridize with nucleic acid molecules of complementary sequence in a sample. A labeled nucleic acid molecule of known sequence can facilitate detection of any complementary molecules in an…

Silver Staining DNA Gels

By National Diagnostics | September 12, 2011 | Comments Off on Silver Staining DNA Gels

Silver staining is more sensitive than ethidium bromide for double stranded DNA, and detects single stranded DNA or RNA with no loss in sensitivity. Silver staining relies on the reduction of silver cations to insoluble…

Ethidium Bromide Staining

By National Diagnostics | September 12, 2011 | Comments Off on Ethidium Bromide Staining

Bands in gels stained with ethidium bromide fluoresce under ultraviolet light. The most commonly used stain for detecting DNA/RNA is ethidium bromide. Ethidium bromide is a DNA interchelator, inserting itself into the spaces between the…

Post-Electrophoretic Visualization with Nuclistain

By National Diagnostics | September 12, 2011 | Comments Off on Post-Electrophoretic Visualization with Nuclistain

National Diagnostics’ Nuclistain offers a significant increase in sensitivity over UV shadowing, along with the convenience of visual staining. With Nuclistain there is no need for UV light. Nuclistain is a blue dye that binds to…

UV Shadowing

By National Diagnostics | September 12, 2011 | Comments Off on UV Shadowing

Detection of DNA/RNA in solution is generally done by measuring its UV absorbance at 260 nm. This absorbance is due to the ring systems in the nitrogen bases, and can also be used for low…

Isoelectric Focusing

By National Diagnostics | September 9, 2011 | Comments Off on Isoelectric Focusing

Conventional electrophoresis techniques can separate up to 100 different proteins on one run. Typically, cell or tissue extracts contain thousands of proteins, most of which will not be resolved into single bands using a separation…