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Electrophoresis Articles

The Polyacrylamide Matrix

By National Diagnostics | August 12, 2011 | Comments Off on The Polyacrylamide Matrix

Polyacrylamide gels are formed by the polymerization of acrylamide in an aqueous solution in the presence of small amounts of a bifunctional crosslinker. The crosslinker is usually methylenebisacrylamide (bis, or MBA). Polyacrylamide gels are formed…

The Electrophoresis Matrix

By National Diagnostics | August 12, 2011 | Comments Off on The Electrophoresis Matrix

In-gel electrophoresis the matrix forces sample components to separate by size, as they move through its porous structure. The matrix provides greater resistance to the movement of larger molecules. It also performs additional functions including…

The Mechanical and Electrical Dynamics of Gel Electrophoresis – Ohm’s Law

By National Diagnostics | August 10, 2011 | Comments Off on The Mechanical and Electrical Dynamics of Gel Electrophoresis – Ohm’s Law

Ohm’s Law: Relationships between electrical parameters Ohm’s law describes the relationship between the voltage, V, the current, I, and the resistance, R, in a DC circuit. A greater voltage produces a greater proportional current through…

The Mechanical and Electrical Dynamics of Gel Electrophoresis — Electrophoresis System Dynamics

By National Diagnostics | August 10, 2011 | Comments Off on The Mechanical and Electrical Dynamics of Gel Electrophoresis — Electrophoresis System Dynamics

Electrophoresis System Dynamics The apparatus in gel electrophoresis constitutes an electrical/thermodynamic system. The apparatus receives energy from the power source and releases energy as heat. The figure below shows a stylized representation of a typical…

The Mechanical and Electrical Dynamics of Gel Electrophoresis – Intro and Sample Mobility

By National Diagnostics | August 10, 2011 | Comments Off on The Mechanical and Electrical Dynamics of Gel Electrophoresis – Intro and Sample Mobility

The term “electrophoresis” refers simply to the movement of particles by an electric force. The first electrophoresis experiments were carried out on molecules in a conductive buffer solution, where the only force acting on the…

Biological Macromolecules – Proteins

By National Diagnostics | August 10, 2011 | Comments Off on Biological Macromolecules – Proteins

Proteins Like nucleic acids, proteins are polymers. While with nucleic acids the repeating unit is the nucleotide, with proteins, the analogous repeating unit is the amino acid. Amino acids consist of a central carbon that…

Biological Macromolecules: Nucleic Acids

By National Diagnostics | August 8, 2011 | Comments Off on Biological Macromolecules: Nucleic Acids

Nucleic Acids Like many biological molecules nucleic acids are polymers, long molecules formed of repeating units. With nucleic acids, the repeating unit is the nucleotide. A nucleotide consists of a five carbon sugar, a nitrogen…

Denaturing Protein Electrophoresis: SDS-PAGE

By National Diagnostics | July 21, 2011 | Comments Off on Denaturing Protein Electrophoresis: SDS-PAGE

In their native form, proteins fold into a variety of shapes, some compact, some elongated. The rate of migration of native proteins through a sieving medium is therefore more a reflection of their relative compactness,…