Electrophoresis

Buffer Additives-Hydrogen Bonding Agents

In most forms of electrophoresis the solution perfusing the gel matrix typically contains one or more substances in addition to the buffer salts.

Buffer Additives-Reducing Agents

Disulfide bonds between or within sample protein molecules can lead to the formation of aggregates as well as play a role in the binding of the sub

Mechanism of Immunostaining

The highly specific binding interaction between antibodies and their unique antigens has been exploited to create sensitive and specific detection

The Polyacrylamide Matrix-Buffer Strength

The buffer system in electrophoresis controls the pH of the gel, preventing damage to sample molecules and, in certain cases, controlling the ioniz

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