Post Electrophoretic Analysis

Autoradiography

Autoradiography is the use of X-ray (or occasionally photographic) film to detect radioactive materials.

Autoradiographic Enhancement with Autofluor

Protein Fixation on Gels

Fixing (or fixation) is the process whereby proteins are denatured and precipitated in large insoluble aggregates within the gel matrix.

Staining Protein Gels with Coomassie Blue

The Coomassie dyes (R-250 and

Staining Proteins Immobilized on Membranes

Immunological detection of proteins requires that proteins be transferred and immobilized onto a membrane support after electrophoresis (see 

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