Protein Detection

Enzyme Linked Immunosorbent Assay (ELISA)

One of the most straightforward applications of immunological detection is the ELISA, or enzyme linked immunosorbent assay.

Overview of Western Blotting

Proteins can also be detected immunologically following electrophoresis, a technique known as Western blotting.

Protein Fixation on Gels

Fixing (or fixation) is the process whereby proteins are denatured and precipitated in large insoluble aggregates within the gel matrix.

Staining Protein Gels with Coomassie Blue

The Coomassie dyes (R-250 and

Staining Proteins Immobilized on Membranes

Immunological detection of proteins requires that proteins be transferred and immobilized onto a membrane support after electrophoresis (see 

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