Guide Strip Technique

In certain instances, the effects of staining a protein may interfere with subsequent analysis. Examples are Coomassie staining when enzymatic activity is required, or silver staining prior to amino acid analysis, when covalent modification of the amino acids will give spurious results. In these cases, it is common to use a “guide strip”. A guide strip is a lane which is run parallel to the lane to be analyzed, and containing either size markers or a duplicate sample. After the gel is run, the guide strip is cut off and stained, and then realigned with the gel and used as a template to guide band excision. The technique is straightforward. The only common error is to fail to re-equilibrate the gel with running buffer after staining. As many stains cause shrinkage or swelling of the gel, re-equilibration is necessary for accurate and consistent realignment.

Guide Strip Technique
In the Guide Strip Technique, a parallel lane is excised and stained to guide band excision.

 

NEXT TOPIC: Staining Proteins Immobilized on Membranes

Shopping cart

0 Items $0.00