Alkaline Blotting
Alkaline Blotting: The Procedure
NORTHERN BLOTTING Follow the basic procedure given in the standard protocol for Northern Blotting, using 40mM NaOH in place of the 10X SSC. SOUTHERN BLOTTING Follow the procedure in the standard protocol for Southern Blotting, omitting Step 5 and using 0.4M NaOH in place of the 10X SSC. |
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