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Gel Electrophoresis of Proteins Articles

Gel Electrophoresis of RNA & Post Electrophoretic Analysis

By National Diagnostics | September 2, 2011 | Comments Off on Gel Electrophoresis of RNA & Post Electrophoretic Analysis

Agarose electrophoresis of RNA requires the inclusion of denaturing agents in the gel. In the absence of denaturants, RNA assumes compact secondary structures, which distort the relationship between molecular weight and mobility.Urea, used as a…


By National Diagnostics | August 12, 2011 | Comments Off on Isotachophoresis

Isotachophoresis is a method of electrophoresis that employs the basic principles of the stacking gel phase of multiphasic systems discussed in the preceding section. Employing nonsieving media, often low percentage polyacrylamide, isotachophoresis in its simplest…

Denaturing Protein Electrophoresis: SDS-PAGE

By National Diagnostics | July 21, 2011 | Comments Off on Denaturing Protein Electrophoresis: SDS-PAGE

In their native form, proteins fold into a variety of shapes, some compact, some elongated. The rate of migration of native proteins through a sieving medium is therefore more a reflection of their relative compactness,…