Denaturing Protein Electrophoresis- SDS PAGE

Sample Preparation for SDS-PAGE

SDS is a powerful detergent, which will solubilize many cells and tissues.

Gel Preparation for SDS-PAGE

Two categories of buffer systems are available for SDS PAGE: continuous and

Casting Gradient Gels

Gradient gels are cast with a higher concentration of acrylamide at the bottom than the top.

Measuring Molecular Weight with SDS-PAGE

The mobility (Rf) of a molecule in gel electrophoresis is determined by its free solution mobility, Y0 (= mobility in a gel o

Peptide Mapping

Peptide mapping involves controlled cleavage of a pure protein with small amounts of a pure protease to generate peptides of characteristic, reprod

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