The electrophoretic analysis of single stranded nucleic acids is complicated by the secondary structures assumed by these molecules.
DNA samples for denaturing gel electrophoresis must be denatured prior to loading, to avoid time dependent denaturation artifacts on the gel.
Sequencing gels are poured between two glass plates separated by spacers. The spacers are typically no more than 0.2mm in thickness.
Molecular weight determination is the most basic use of denaturing polyacrylamide gel electrophoresis.
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