News

January 15, 2014
Faster Westerns with Protogel Quick Cast

October 4, 2013
National Diagnostics introduces new products for genomic electrophoresis

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012
ProtoGlow ECL Launched

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

Search results

  • Articles : Denaturing Polyacrylamide Gel Electrophoress of DNA and RNA

Denaturing Polyacrylamide Gel Electrophoress of DNA and RNA

Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA

The electrophoretic analysis of single stranded nucleic acids is complicated by the secondary structures assumed by these molecules.

Sample Prep for Denaturing PAGE of DNA

DNA samples for denaturing gel electrophoresis must be denatured prior to loading, to avoid time dependent denaturation artifacts on the gel.

Preparing Denaturing DNA & RNA Gels

Sequencing gels are poured between two glass plates separated by spacers. The spacers are typically no more than 0.2mm in thickness.

Run Conditions in Denaturing PAGE

Temperature

Using PAGE to Determine Nucleic Acid Molecular Weight

Molecular weight determination is the most basic use of denaturing polyacrylamide gel electrophoresis.

Pages

Shopping cart

0 Items $0.00