Denaturing Polyacrylamide Gel Electrophoress of DNA and RNA

Denaturing Polyacrylamide Gel Electrophoresis of DNA & RNA

The electrophoretic analysis of single stranded nucleic acids is complicated by the secondary structures assumed by these molecules.

Sample Prep for Denaturing PAGE of DNA

DNA samples for denaturing gel electrophoresis must be denatured prior to loading, to avoid time dependent denaturation artifacts on the gel.

Preparing Denaturing DNA & RNA Gels

Sequencing gels are poured between two glass plates separated by spacers. The spacers are typically no more than 0.2mm in thickness.

Using PAGE to Determine Nucleic Acid Molecular Weight

Molecular weight determination is the most basic use of denaturing polyacrylamide gel electrophoresis.


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