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  • Articles : Agarose Gel Electrophoresis of DNA and RNA

Agarose Gel Electrophoresis of DNA and RNA

Restriction Digest Mapping

Restriction endonucleases are enzymes that cleave double stranded DNA at specific sites, generally 4, 6 or 8 base palindromic sequences.

DNA/RNA Purification from Agarose Gels - Electroelution

The most popular alternative to glass powder elution for the complete purification of DNA from agarose is electroelution.

In Gel Enzyme Reactions

In many cases, the processing of DNA by enzymes is not impeded by agarose.

PFGE and FIGE

Standard agarose gels can resolve DNA fragments up to 75 kb. Often, analysis of genomic DNA requires resolution of megabase (mb) fragments.

RNA Electrophoresis

Electrophoretic analysis of RNA presents unique challenges. RNA is isolated in single stranded form, without complementary sequences.

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