Restriction endonucleases are enzymes that cleave double stranded DNA at specific sites, generally 4, 6 or 8 base palindromic sequences.
The most popular alternative to glass powder elution for the complete purification of DNA from agarose is electroelution.
In many cases, the processing of DNA by enzymes is not impeded by agarose.
Standard agarose gels can resolve DNA fragments up to 75 kb. Often, analysis of genomic DNA requires resolution of megabase (mb) fragments.
Electrophoretic analysis of RNA presents unique challenges. RNA is isolated in single stranded form, without complementary sequences.
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