October 4, 2013

National Diagnostics continues its tradition of bringing laboratories more high quality options for scientific research by introducing three new p

July 23, 2012

National Diagnostics announces the release of a new, ultra-high sensitivity reagent for HRP mediated Western Blotting.

Conformational Analysis

Native DNA PAGE gels can be used to detect small mutational differences between DNA molecules. In heteroduplex analysis, for double stranded DNA, the basis of separation is the conformational difference arising from the bending of the rodlike double helix caused by small mismatches between the strands. In SSCP analysis, single stranded DNA molecules are fractionated based on the compactness of their folded structure. In both cases, it is possible to separate strands of equal length which have different sequences, often differing by only one base. Given a control (unmutated) fragment to use as a gel standard, these techniques can be used to screen large numbers of samples for mutations.

NEXT TOPIC: Heteroduplex Analysis

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