Native PAGE gels are prepared by mixing an acrylamide/bisacrylamide monomer concentrate (AccuGel 19:1 or 29:1), buffer concentrate, and water to achieve the desired gel concentration. TEMED and ammonium persulfate are added to initiate polymerization and the solution is poured into a cassette. The comb is then inserted.

The cassette is formed by two glass plates separated by spacers, typically 0.5-1mm in thickness, and sealed at the bottom (See figure below or follow this link for a more detailed discussion of the electrophoresis apparatus). The polymerization process takes 1-2 hours to complete.

The table below gives solution amounts to prepare gels of various percentages and buffers.

Instructions:

1. Prepare a solution of monomer, buffer, and water as per the table.
2. De-gas if desired.
3. Add APS and TEMED.
4. Pour gel.

Formulas for Native DNA Polyacrylamide Gels using National Diagnostics’ AccuGel 40%

Gel %	TBE Gels	TAE Gels	TTE Gels
6	AccuGel: 15 mL 10X TBE: 10 mL Deionized H2O: 75 mL	AccuGel: 15 mL 50X TAE: 2 mL Deionized H2O: 83 mL	AccuGel: 15 mL 20X TTE: 5 mL Deionized H2O: 80 mL
8	AccuGel: 20 mL 10X TBE: 10 mL Deionized H2O: 70 mL	AccuGel: 20 mL 50X TAE: 2 mL Deionized H2O: 87 mL	AccuGel: 20 mL 20X TTE: 5 mL Deionized H2O: 75 mL
10	AccuGel: 25 mL 10X TBE: 10 mL Deionized H2O: 65 mL	AccuGel: 25 mL 50X TAE: 2 mL Deionized H2O: 78 mL	AccuGel: 25 mL 20X TTE: 5 mL Deionized H2O: 70 mL
12	AccuGel: 30 mL 10X TBE: 10 mL Deionized H2O: 60 mL	AccuGel: 30 mL 50X TAE: 2 mL Deionized H2O: 73 mL	AccuGel: 30 mL 20X TTE: 5 mL Deionized H2O: 65 mL
14	AccuGel: 35 mL 10X TBE: 10 mL Deionized H2O: 55 mL	AccuGel: 35 mL 50X TAE: 2 mL Deionized H2O: 68 mL	AccuGel: 35 mL 20X TTE: 5 mL Deionized H2O: 60 mL

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