In most forms of electrophoresis the solution perfusing the gel matrix typically contains one or more substances in addition to the buffer salts.
Disulfide bonds between or within sample protein molecules can lead to the formation of aggregates as well as play a role in the binding of the sub
In its simplest form, a buffered solution contains a mixture of a weak acid and its conjugate base.
Several factors to consider when choosing a buffer include:
In a homogeneous buffer system, the identity and concentration of buffer components are the same in the gel and the tanks.
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