The Buffer

Buffer Additives-Hydrogen Bonding Agents

In most forms of electrophoresis the solution perfusing the gel matrix typically contains one or more substances in addition to the buffer salts.

Buffer Additives-Reducing Agents

Disulfide bonds between or within sample protein molecules can lead to the formation of aggregates as well as play a role in the binding of the sub

Electrophoresis Buffers--The Henderson-Hasselbalch Equation

In its simplest form, a buffered solution contains a mixture of a weak acid and its conjugate base.

Electrophoresis Buffers-Choosing the Right Buffer

Several factors to consider when choosing a buffer include:

Homogeneous Buffer Systems

In a homogeneous buffer system, the identity and concentration of buffer components are the same in the gel and the tanks.

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