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Oxygen Assays

Hydrogen Peroxide Assay Kit

$187.00


Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.
 

Catalog Number: CL-204

  • Sensitive, Quantitative Assay for Hydrogen Peroxide
  • Detects as Little as 15ng/ml
  • Easy-to-use, Colorimetric System
SKU: CL-204 Categories: ,

Description


Hazardous Shipping Fees: This product may incur an additional hazardous shipping fee. We will pack the products to minimize all shipping costs, and the final charge will reflect the shipping cost and any applicable hazardous fees charged by FedEx.
 
Catalog Number: CL-204
  • Sensitive, Quantitative Assay for Hydrogen Peroxide
  • Detects as Little as 15ng/ml
  • Easy-to-use, Colorimetric System

Hydrogen peroxide is a part of the oxygen reduction pathway, produced by the two-electron reduction of molecular oxygen, or by the one-electron reduction of superoxide anion radical. Hydrogen peroxide is a potent oxidant, and the levels of hydrogen peroxide must be accurately determined in order to fully characterize the oxidative state of the system under study.

National Diagnostics Hydrogen Peroxide Assay Kit is a rapid, sensitive, and quantitative method for the determination of Hydrogen Peroxide in chemical or biological systems. The assay is based upon formation of a complex between Xylenol Orange and ferric iron, which is produced by the peroxide dependent oxidation of ferrous iron. This reaction is quantified colorimetrically, detecting as little as 15ng/ml of peroxide. Linear results can be obtained up to approximately 1000ng/ml.

National Diagnostics Hydrogen Peroxide Assay is the ideal complement to our well established Diogenes Superoxide detection system. Using these kits in tandem, researchers can now quantitatively determine the first two steps in the oxygen reduction pathway.

Additional information

Weight 2 lbs
Dimensions 8 × 3 × 10 in

Protocol

Hydrogen Peroxide Assay Protocol

Assay Reagent Preparation
Prepare 1.8ml of reagent per sample to run tests in duplicate.
1. To prepare 20ml: Combine 19.8ml of Component A with 0.2ml
Component B.
2. The mixture is stable at room temperature for one working day.

Assay Procedure
1. Mix 0.9ml of Assay Reagent with up to 0.1ml of sample.
2. Incubate at room temperature for at least 30 minutes to allow
for complete color development.
3. Read absorbance at 560nm.

Catalog Number: CL-204 Download Full Protocol PDF >

Safety Overview

Hydrogen Peroxide Assay Reagent A Safety Overview

Catalog Number: CL-204A
Safety Summary (see MSDS for complete information before using the product):

Safety Summary (see MSDS for complete information before using the product): 

Appearance and Odor

Dark, straw-colored liquid.

EMERGENCY OVERVIEW – IMMEDIATE HAZARD

SORBITOL

HIGH CONCENTRATIONS MAY IRRITATE EYES AND RESPIRATORY TRACT.

XYLENOL ORANGE

Hazardous in case of skin contact (irritant), of eye contact (irritant), of ingestion, of inhalation.

EMERGENCY OVERVIEW – CHRONIC HAZARD WARNING:

SORBITOL

AS PART OF GOOD LABORATORY AND PERSONAL HYGIENE AND SAFETY PROCEDURE, AVOID ALL UNNECESSARY EXPOSURE TO THE CHEMICAL SUBSTANCE AND ENSURE PROMPT REMOVAL FROM SKIN, EYES, AND CLOTHING.

XYLENOL ORANGE

Repeated or prolonged exposure is not known to aggravate medical condition.


Hydrogen Peroxide Assay Reagent B Safety Overview

Catalog Number: CL-204B
Safety Summary (see MSDS for complete information before using product):
 
Toxic by inhalation, in contact with skin and if swallowed
Avoid contact with skin and eyes
EMERGENCY OVERVIEW – IMMEDIATE HAZARD
TOXIC BY INHALATION, IN CONTACT WITH SKIN AND IF SWALLOWED.

Full MSDS

Diogenes Activator: Full MSDS
Catalog Number: CL-204B
View & Download PDF > 


Diogenes Reagent: Full MSDS
Catalog Number: CL-204A
View & Download PDF >