Radiation Safety
Here are a few guidelines which you can use to process fixed tissue using Histo-Clear and Histo-Clear II: Histo-Clear Automated Tissue Processing Schedule Process Bath 18 Hour Cycle (Time in Hours) 24 Hour Cycle (Time…
Particulate in the sample: Standard loading buffers are often unable to completely dissolve complex samples- fragments of bacterial cell walls, for example, can be carried over into the well. Particulate material in the well blocks…
This gel can be recovered in most cases by fully destaining in methanol:water: acetic acid, followed by a fresh round of staining. In severe cases, the deposits may be removed by gently spraying the gel…
Uneven staining is almost always the result of insufficient agitation during staining. The gel is initially more dense than the staining solution and will tend to sink to the bottom of the dish. Some portions…
A high background that obscures the bands, or in combination with fainter than usual bands, indicates dye binding to the gel matrix, or contamination of the matrix with a dye-binding material (most often a protein).…
SDS in the staining solution: Most users save and re-use their staining solutions. This is fine for a couple of cycles, but over time enough SDS elutes from the gels to accumulate to significant levels…
If your gel doesn’t look like this one, click on the problem below to find the solution: Faint bands on a low background Learn More Faint bands on a high background Learn More Uneven Staining…
The OptiClear Solvents OptiClearOE-101 OptiClear ROE-102 OptiClear EOE-104 OptiClear SOE-105 OptiClear S2OE-107 OptiClear WOE-106 Flash Point (oF) 120 142 45 147 177 199 Non-Toxic? Yes Yes Yes Yes Yes Yes Biodegradable? Yes Yes Yes…
Prior to the introduction of liquid scintillation counting, a primary route of radiotracer analysis was to combust the organic material and detect the 14CO2 so generated in a gas phase proportional counter. Many protocols still…
Most research applications of radioisotopes require an eventual quantitation of the isotope, which is done by measuring the intensity of radiation emitted. Common nomenclature expresses this intensity as disintegrations per minute (DPM). The Becquerel (Bq)…