Diogenes

  • Cellular Luminescence Enhancement System for Superoxide Detection
  • Easy to Use - Specific for Superoxide
  • From 100-Fold to 600-Fold Enhancement
  • Requires Fewer Cells - Non Cytotoxic
Catalog number: CL-202

Diogenes Cellular Luminescence Enhancement System is a superoxide chemiluminescent enhancer that is non-denaturing to living cells. Superoxide radical (O2-) is produced intracellularly as a consequence of aerobic metabolism and extracellularly by leukocytes in response to infection. The extent of oxidative burst produced by white blood cells (WBCs) when stimulated by f-met-leu-phe, phorbol esters, anti-Fc receptor antibodies or LPS is a partial indicator of the immunocompetence of the cells tested.

Currently, the production of O2- by leukocytes is monitored by such cumbersome and indirect methods as measuring oxygen uptake in a Clark electrode (both in the presence and absence of cyanide) or measuring spectral changes caused by the reduction of cytochrome c. As a non-cytotoxic intermediate in the mechanism of photon production, Diogenes is ideally suited to the detection of cell-mediated superoxide production. The intensity of light produced by Diogenes in the presence of superoxide is directly proportional to the O2- concentration, but is much higher than that achieved by using luminol. Therefore, Diogenes is ideal for monitoring cellular immunocompetence, utilizing a luminometer to quantify the light output. Any stimulant that activates an oxidase to produce extracellular superoxide is usable with Diogenes. Such means can be physiological ormimetic of the physiologic pathway.

 

Diogenes References:

Tsuyoshi Yamazaki, Chikage Kawai, [...], and Futoshi Kuribayashi
Trop Med Health. 2011 June; 39(2): 41–45.

 

Miklós Geiszt, Jeffrey B. Kopp, [...], and Thomas L. Leto
Proc Natl Acad Sci U S A. 2000 July 5; 97(14): 8010–8014.

 

Futoshi Kuribayashi1, Hiroyuki Nunoi, Kaori Wakamatsu, Shohko Tsunawaki, Kazuki Sato, Takashi Ito and Hideki Sumimoto
EMBO Journal (2002) 21, 6312 - 6320 

 

     

 

Protocol: 

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