Fundamentals of Liquid Scintillation Counting ARTICLES
For mechanical support during the sectioning process, tissue must be infiltrated with an embedding medium. The usual embedding media are paraffin for light microscopy and an epoxy resin for EM samples. Paraffins are available that…
The step following dehydration is called “clearing” and consists of replacing the dehydrant with a substance that will be miscible with the embedding medium (paraffin). The term “clearing” comes from the fact that the clearing…
Dehydration is usually carried out by transferring the tissue through solutions of increasing alcohol concentration, until 100% alcohol is reached. Sometimes the first step is a mixture of formalin and alcohol. Other dehydrants can be…
Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope. The procedures designed to prepare the tissue for sectioning are collectively known as tissue…
The removal of calcium deposits is essential for good embedding procedure. Decalcification is usually carried out between the fixation and processing steps. Bone must obviously be processed in this way, but other tissues may also…
ELECTROPHORESIS Prepare and run an SDS PAGE gel. Select a gel percent which will give the best resolution for the size of antigen being analyzed (if known). If the size is not known, a 12%…
Fixatives are among the most hazardous substances used in life science research. Work with these substances under the hood wearing gloves, lab coat and safety goggles. Formaldehyde is a suspect cancer hazard and a strong…
Fixation protocols are usually straightforward. The tissue is cut to dimensions suited to the rate of penetration of the particular fixative and placed in the fixative solution. The number of factors affecting the fixation process…
Mercury Based Fixatives Mercurials contain mercuric chloride. Their method of tissue fixation is poorly understood. While not penetrating tissue well and causing some tissue hardness, mercurials are fast and provide excellent nuclear detail. They are…
Liquid scintillation counting of discrete samples is conceptually straightforward. A sample is mixed with an appropriate volume of scintillation cocktail, and the mixture is placed in an LSC vial and counted. For some samples no…
- Waste Disposal Issues in Scintillation Counting
- The Complete Scintillation Cocktail
- Radioactive Emissions and the Use of Isotopes in Research
- Preparing Tissue Samples for Scintillation Counting
- Preparing Samples in PAGE Gels for LSC
- Mechanism of Liquid Scintillation Counting
- Measurement of Radiation and Isotope Quantitation
- Liquid Scintillation Signal Interpretation
- Liquid Scintillation and Radiation Safety
- HPLC Flow Counting
- Counting Samples on Cellulose-Ester Filters
- Counting Samples from TLC Plates by LSC
- Counting Efficiency and Quenching
- Counting Carbon Dioxide by LSC
- Chemiluminescence and Static Electricity
- Assaying Discrete Samples by Liquid Scintillation Counting