Protein Detection
Particulate in the sample: Standard loading buffers are often unable to completely dissolve complex samples- fragments of bacterial cell walls, for example, can be carried over into the well. Particulate material in the well blocks…
Blotches can be caused by overhandling of the gel, or by handling the gel without gloves. This can create uneven surfaces or protein deposits that can bind Coomassie to the gel. To minimize this problem,…
Uneven staining is almost always the result of insufficient agitation during staining. The gel is initially more dense than the staining solution and will tend to sink to the bottom of the dish. Some portions…
If your gel doesn’t look like this one, click on the problem below to find the solution: Faint bands on a low background Learn More Faint bands on a high background Learn More Uneven Staining…