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Oxygen Assays Articles

Hydrogen Peroxide Detection: The Methods

By National Diagnostics | October 10, 2011 | Comments Off on Hydrogen Peroxide Detection: The Methods

In at least one important way, measuring hydrogen peroxide is substantially easier than measuring superoxide. Superoxide is unstable in aqueous solution—its steady state concentration cannot be measured directly. As a result, superoxide “levels” must be…

Hydrogen Peroxide Detection: An Overview

By National Diagnostics | October 10, 2011 | Comments Off on Hydrogen Peroxide Detection: An Overview

Hydrogen peroxide, like superoxide, can react with a variety of targets in the cell, and has been associated with a number of diseases. Aerobic organisms express catalase and peroxidase enzymes to prevent damage by H2O2.…

Superoxide Detection

By National Diagnostics | October 10, 2011 | Comments Off on Superoxide Detection

Overview Superoxide has been implicated in diseases ranging from Alzheimer’s to diabetes. The wide range of pathologies associated with superoxide is the result of its ability to react with a variety of cellular targets, including…

Reactive Oxygen Species

By National Diagnostics | October 7, 2011 | Comments Off on Reactive Oxygen Species

Oxygen is used by a great variety of organisms as a means for producing energy. The redox potential of the oxygen-water couple is 1.229 Volts, meaning that a relatively large amount of energy is released…

Immunostaining with Horseradish Peroxidase

By National Diagnostics | September 19, 2011 | Comments Off on Immunostaining with Horseradish Peroxidase

Chromogenic and luminometric substances are also available for horseradish peroxidase (HRP). The “classic” chromogen used with HRP is diaminobenzidine (DAB). In the presence of H2O2, HRP will oxidize DAB, creating a water insoluble brown precipitate.…