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Histology Fundamentals

Decalcifying Tissue for Histological Processing

The removal of calcium deposits is essential for good embedding procedure.

Overview of the Paraffin Technique

Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope.

Dehydration

Dehydration is usually carried out by transferring the tissue through solutions of increasing alcohol concentration, until 100% alcohol is reached

Clearing Tissue Sections

The step following dehydration is called "clearing" and consists of replacing the dehydrant with a substance that will be miscible with the embeddi

Embedding

For mechanical support during the sectioning process, tissue must be infiltrated with an embedding medium.

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