HISTOLOGY FUNDAMENTALS Articles
The removal of calcium deposits is essential for good embedding procedure. Decalcification is usually carried out between the fixation and processing steps. Bone must obviously be processed in this way, but other tissues may also…
Fixatives are among the most hazardous substances used in life science research. Work with these substances under the hood wearing gloves, lab coat and safety goggles. Formaldehyde is a suspect cancer hazard and a strong…
Fixation protocols are usually straightforward. The tissue is cut to dimensions suited to the rate of penetration of the particular fixative and placed in the fixative solution. The number of factors affecting the fixation process…
Mercury Based Fixatives Mercurials contain mercuric chloride. Their method of tissue fixation is poorly understood. While not penetrating tissue well and causing some tissue hardness, mercurials are fast and provide excellent nuclear detail. They are…
Formaldehyde and glutaraldehyde are the most commonly used aldehyde fixatives. They work by forming cross-links both within and between proteins, particularly between lysine residues. Damage to the tertiary structure of the proteins occurs on a…
To maintain the tissue in as lifelike a state as possible, tissue for analysis is usually placed directly into a fixative solution upon removal from the body. Fixation is normally carried out as soon as…
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- Working Safely with Fixatives
- The Chemistry of Dyes and Staining
- Suggested procedures for processing fixed tissue
- Staining Procedures
- Sectioning
- Overview of the Paraffin Technique
- Overview of Fixation
- Non-Aldehyde Fixatives
- Mounting Tissue Sections
- Factors Affecting Fixation
- Embedding
- Dehydration
- Decalcifying Tissue for Histological Processing
- Clearing Tissue Sections
- Artifacts in Histologic Sections
- Aldehyde Fixatives