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HISTOLOGY FUNDAMENTALS Articles

Decalcifying Tissue for Histological Processing

By National Diagnostics | September 26, 2011 | Comments Off on Decalcifying Tissue for Histological Processing

The removal of calcium deposits is essential for good embedding procedure. Decalcification is usually carried out between the fixation and processing steps. Bone must obviously be processed in this way, but other tissues may also…

Working Safely with Fixatives

By National Diagnostics | September 22, 2011 | Comments Off on Working Safely with Fixatives

Fixatives are among the most hazardous substances used in life science research. Work with these substances under the hood wearing gloves, lab coat and safety goggles. Formaldehyde is a suspect cancer hazard and a strong…

Factors Affecting Fixation

By National Diagnostics | September 22, 2011 | Comments Off on Factors Affecting Fixation

Fixation protocols are usually straightforward. The tissue is cut to dimensions suited to the rate of penetration of the particular fixative and placed in the fixative solution. The number of factors affecting the fixation process…

Non-Aldehyde Fixatives

By National Diagnostics | September 22, 2011 | Comments Off on Non-Aldehyde Fixatives

Mercury Based Fixatives   Mercurials contain mercuric chloride. Their method of tissue fixation is poorly understood. While not penetrating tissue well and causing some tissue hardness, mercurials are fast and provide excellent nuclear detail. They…

Aldehyde Fixatives

By National Diagnostics | September 20, 2011 | Comments Off on Aldehyde Fixatives

Formaldehyde and glutaraldehyde are the most commonly used aldehyde fixatives. They work by forming cross-links both within and between proteins, particularly between lysine residues. Damage to the tertiary structure of the proteins occurs on a…

Overview of Fixation

By National Diagnostics | September 20, 2011 | Comments Off on Overview of Fixation

To maintain the tissue in as lifelike a state as possible, tissue for analysis is usually placed directly into a fixative solution upon removal from the body. Fixation is normally carried out as soon as…