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Tissue Preparation

Overview of the Paraffin Technique

Once fixed, the tissue must be treated to allow the cutting of the thin sections required for viewing under the microscope.

Dehydration

Dehydration is usually carried out by transferring the tissue through solutions of increasing alcohol concentration, until 100% alcohol is reached.

Suggested procedures for processing fixed tissue

Here are a few guidelines which you can use to process fixed tissue using Histo-Clear and Histo-Clear II:

Clearing Tissue Sections

The step following dehydration is called "clearing" and consists of replacing the dehydrant with a substance that will be miscible with the embeddi

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