Native Polyacrylamide Electrophoresis of DNA and RNA

Native PAGE of DNA

In the absence of denaturants double stranded DNA retains its double helical structure, which gives it a rodlike form as it migrates through a gel

Gel Preparation for Native PAGE of DNA

Native PAGE gels are prepared by mixing an acrylamide/bisacrylamide monomer concentrate (AccuGel

PCR Analysis: An Examination

The polymerase chain reaction (PCR) is a powerful technique which uses repetitive cycles of primer annealing, primer extension, and product denatu

PCR Analysis: Yield and Kinetics

PCR reactions produce product in a nonlinear pattern (See figure below).


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